An ice-binding protein from an Arctic grass, Leymus mollis

Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass that had not previously been examined, Leymus mollis (Pooidae). Rhizome tissue had strong ice-structuring and ice recry...

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Bibliographic Details
Published in:F1000Research
Main Authors: Sformo, Todd L., Raymond, James A.
Format: Text
Language:English
Published: F1000 Research Limited 2020
Subjects:
Brief Report
Arctic
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383708/
https://doi.org/10.12688/f1000research.24328.1
Description
Summary:Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass that had not previously been examined, Leymus mollis (Pooidae). Rhizome tissue had strong ice-structuring and ice recrystallization inhibiting (IRI) activities, indicating the probable presence of IBPs. The gene sequence of an IBP was obtained. The sequence encoded a 118-amino acid IRI domain that contained eight repeats. A 3D structure of the IRI domain was predicted from the structure of the IRI domain of the perennial ryegrass Lolium perenne. The predicted structure appeared to have the same eight beta-roll coils found in the L. perenne IBP.

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