Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahy...

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Published in:Frontiers in Bioengineering and Biotechnology
Main Authors: Wang, Yangxin, Zhang, Ningning, Tan, Deming, Qi, Zhenhui, Wu, Changzhu
Format: Text
Language:English
Published: Frontiers Media S.A. 2020
Subjects:
Bioengineering and Biotechnology
Antarc*
Antarctica
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/
https://doi.org/10.3389/fbioe.2020.00714
id ftpubmed:oai:pubmedcentral.nih.gov:7358279
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spelling ftpubmed:oai:pubmedcentral.nih.gov:7358279 2023-05-15T13:34:38+02:00 Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent Wang, Yangxin Zhang, Ningning Tan, Deming Qi, Zhenhui Wu, Changzhu 2020-07-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/ https://doi.org/10.3389/fbioe.2020.00714 en eng Frontiers Media S.A. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/ http://dx.doi.org/10.3389/fbioe.2020.00714 Copyright © 2020 Wang, Zhang, Tan, Qi and Wu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. CC-BY Front Bioeng Biotechnol Bioengineering and Biotechnology Text 2020 ftpubmed https://doi.org/10.3389/fbioe.2020.00714 2020-08-02T00:19:41Z In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO(3))(2)⋅6H(2)O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability. Text Antarc* Antarctica PubMed Central (PMC) Frontiers in Bioengineering and Biotechnology 8
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Bioengineering and Biotechnology
spellingShingle Bioengineering and Biotechnology
Wang, Yangxin
Zhang, Ningning
Tan, Deming
Qi, Zhenhui
Wu, Changzhu
Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
topic_facet Bioengineering and Biotechnology
description In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO(3))(2)⋅6H(2)O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.
format Text
author Wang, Yangxin
Zhang, Ningning
Tan, Deming
Qi, Zhenhui
Wu, Changzhu
author_facet Wang, Yangxin
Zhang, Ningning
Tan, Deming
Qi, Zhenhui
Wu, Changzhu
author_sort Wang, Yangxin
title Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_short Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_full Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_fullStr Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_full_unstemmed Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent
title_sort facile synthesis of enzyme-embedded metal–organic frameworks for size-selective biocatalysis in organic solvent
publisher Frontiers Media S.A.
publishDate 2020
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/
https://doi.org/10.3389/fbioe.2020.00714
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_source Front Bioeng Biotechnol
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/
http://dx.doi.org/10.3389/fbioe.2020.00714
op_rights Copyright © 2020 Wang, Zhang, Tan, Qi and Wu.
http://creativecommons.org/licenses/by/4.0/
This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
op_rightsnorm CC-BY
op_doi https://doi.org/10.3389/fbioe.2020.00714
container_title Frontiers in Bioengineering and Biotechnology
container_volume 8
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