Facile Synthesis of Enzyme-Embedded Metal–Organic Frameworks for Size-Selective Biocatalysis in Organic Solvent

In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahy...

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Bibliographic Details
Published in:Frontiers in Bioengineering and Biotechnology
Main Authors: Wang, Yangxin, Zhang, Ningning, Tan, Deming, Qi, Zhenhui, Wu, Changzhu
Format: Text
Language:English
Published: Frontiers Media S.A. 2020
Subjects:
Bioengineering and Biotechnology
Antarc*
Antarctica
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7358279/
https://doi.org/10.3389/fbioe.2020.00714
Description
Summary:In situ immobilization of enzyme into metal–organic frameworks (MOFs) is performed through a one-step and facile method. Candida antarctica lipase B (CalB) is directly embedded in zeolitic imidazolate framework (ZIF)-8 by simply mixing an aqueous solution of 2-methylimidazole and zinc nitrate hexahydrate [Zn(NO(3))(2)⋅6H(2)O] containing CalB at room temperature. Due to the intrinsic micropores of ZIF-8, the obtained CalB@ZIF composite is successfully applied in size-selective transesterification reaction in organic solvent. CalB@ZIF not only shows much higher catalytic activity but also exhibits higher thermal stability than free CalB. Besides, the robust ZIF-8 shell also offers the hybrid composites excellent reusability.

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