Hypoxic acclimation negatively impacts the contractility of steelhead trout (Oncorhynchus mykiss) spongy myocardium
Cardiac stroke volume (S(V)) is compromised in Atlantic cod and rainbow trout following acclimation to hypoxia (i.e., 40% air saturation; ~8 kPa O(2)) at 10–12°C, and this is not due to changes in heart morphometrics or maximum achievable in vitro end-diastolic volume. To examine if this diminished...
| Published in: | American Journal of Physiology-Regulatory, Integrative and Comparative Physiology |
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| Main Authors: | , , , |
| Format: | Text |
| Language: | English |
| Published: |
American Physiological Society
2020
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| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7052596/ http://www.ncbi.nlm.nih.gov/pubmed/31747300 https://doi.org/10.1152/ajpregu.00107.2019 |
| Summary: | Cardiac stroke volume (S(V)) is compromised in Atlantic cod and rainbow trout following acclimation to hypoxia (i.e., 40% air saturation; ~8 kPa O(2)) at 10–12°C, and this is not due to changes in heart morphometrics or maximum achievable in vitro end-diastolic volume. To examine if this diminished S(V) may be related to compromised myocardial contractility, we used the work-loop method to measure work and power in spongy myocardial strips from normoxic- and hypoxic-acclimated steelhead trout when exposed to decreasing Po(2) levels (21 to 1.5 kPa) at several frequencies (30–90 contractions/min) at 14°C (their acclimation temperature). Work required to lengthen the muscle, as during filling of the heart, was strongly frequency dependent (i.e., increased with contraction rate) but was not affected by hypoxic acclimation or test Po(2). In contrast, although shortening work was less frequency dependent, this parameter and network (and power) 1) were consistently lower (by ~30–50 and ~15%, respectively) in strips from hypoxic-acclimated fish and 2) fell by ~40–50% in both groups from 20 to 1.5 kPa Po(2), despite the already-reduced myocardial performance in the hypoxic-acclimated group. In addition, strips from hypoxic-acclimated trout showed a poorer recovery of net power (by ~15%) when returned to normoxia. These results strongly suggest that hypoxic acclimation reduces myocardial contractility, and in turn, may limit S(V) (possibly by increasing end-systolic volume), but that this diminished performance does not improve the capacity to maintain myocardial performance under oxygen limiting conditions. |
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