Flavobacterium petrolei sp. nov., a novel psychrophilic, diesel-degrading bacterium isolated from oil-contaminated Arctic soil
This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42(T) and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that the...
Published in: | Scientific Reports |
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Main Authors: | , , , |
Format: | Text |
Language: | English |
Published: |
Nature Publishing Group UK
2019
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Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6411956/ http://www.ncbi.nlm.nih.gov/pubmed/30858439 https://doi.org/10.1038/s41598-019-40667-7 |
Summary: | This study presents taxonomic description of two novel diesel-degrading, psychrophilic strains: Kopri-42(T) and Kopri-43, isolated during screening of oil-degrading psychrotrophs from oil-contaminated Arctic soil. A preliminary 16S rRNA gene sequence and phylogenetic tree analysis indicated that these Arctic strains belonged to the genus Flavobacterium, with the nearest relative being Flavobacterium psychrolimnae LMG 22018(T) (98.9% sequence similarity). The pairwise 16S rRNA gene sequence identity between strains Kopri-42(T) and Kopri-43 was 99.7%. The DNA-DNA hybridization value between strain Kopri-42(T) and Kopri-43 was 88.6 ± 2.1% indicating that Kopri-42(T) and Kopri-43 represents two strains of the same genomospecies. The average nucleotide identity and in silico DNA-DNA hybridization values between strain Kopri-42(T) and nearest relative F. psychrolimnae LMG 22018(T) were 92.4% and 47.9%, respectively. These values support the authenticity of the novel species and confirmed the strain Kopri-42(T) belonged to the genus Flavobacterium as a new member. The morphological, physiological, biochemical and chemotaxonomic data also distinguished strain Kopri-42(T) from its closest phylogenetic neighbors. Based on the polyphasic data, strains Kopri-42(T) and Kopri-43 represents a single novel species of the genus Flavobacterium, for which the name Flavobacterium petrolei sp. nov. is proposed. The type strain is Kopri-42(T) (=KEMB 9005-710(T) = KACC 19625(T) = NBRC 113374(T)). |
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