Isolation and Characterization of Polysaccharides from Oysters (Crassostrea gigas) with Anti-Tumor Activities Using an Aqueous Two-Phase System
In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be...
| Published in: | Marine Drugs |
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| Main Authors: | , , |
| Format: | Text |
| Language: | English |
| Published: |
MDPI
2017
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| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5706028/ http://www.ncbi.nlm.nih.gov/pubmed/29104211 https://doi.org/10.3390/md15110338 |
| Summary: | In this study, a simple aqueous two-phase system (ATPS) was employed for concurrent purification of oyster polysaccharides. The chemical structure and anti-tumor activities of purified oyster polysaccharides (OP-1) were also investigated. Under optimal ATPS conditions, oyster polysaccharides can be partitioned in the bottom phase with 67.02% extraction efficiency. The molecular weight of OP-1 was determined as 3480 Da. OP-1 is a (1→4)-α-d-glucosyl backbone and branching points located at O-3 of glucose with a terminal-d-Glcp. The anti-tumor activity assay showed that OP-1 exhibited good activities, including promotion of splenocyte proliferation, IL-2 release, and inhibition of HepG2 cell proliferation. Additionally, OP-1 had no in vivo toxicity. This finding suggests that ATPS is a much simpler and greener system, and it opens up new possibilities in the large-scale separation of active polysaccharides from oysters. OP-1 could be used by the health food and pharmaceutical therapies as potential anti-cancer adjuvants. |
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