Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

A Ser105Ala mutant of the lipase B from Candida antarctica enables ‘perhydrolase-only’ reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially elimi...

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Published in:Scientific Reports
Main Authors: Wang, Xu-Ping, Zhou, Peng-Fei, Li, Zhi-Gang, Yang, Bo, Hollmann, Frank, Wang, Yong-Hua
Format: Text
Language:English
Published: Nature Publishing Group 2017
Subjects:
Article
Antarc*
Antarctica
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357956/
http://www.ncbi.nlm.nih.gov/pubmed/28317884
https://doi.org/10.1038/srep44599
id ftpubmed:oai:pubmedcentral.nih.gov:5357956
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spelling ftpubmed:oai:pubmedcentral.nih.gov:5357956 2023-05-15T13:39:48+02:00 Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity Wang, Xu-Ping Zhou, Peng-Fei Li, Zhi-Gang Yang, Bo Hollmann, Frank Wang, Yong-Hua 2017-03-20 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357956/ http://www.ncbi.nlm.nih.gov/pubmed/28317884 https://doi.org/10.1038/srep44599 en eng Nature Publishing Group http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357956/ http://www.ncbi.nlm.nih.gov/pubmed/28317884 http://dx.doi.org/10.1038/srep44599 Copyright © 2017, The Author(s) http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ CC-BY Article Text 2017 ftpubmed https://doi.org/10.1038/srep44599 2017-03-26T01:13:52Z A Ser105Ala mutant of the lipase B from Candida antarctica enables ‘perhydrolase-only’ reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product. Text Antarc* Antarctica PubMed Central (PMC) Scientific Reports 7 1
institution Open Polar
collection PubMed Central (PMC)
op_collection_id ftpubmed
language English
topic Article
spellingShingle Article
Wang, Xu-Ping
Zhou, Peng-Fei
Li, Zhi-Gang
Yang, Bo
Hollmann, Frank
Wang, Yong-Hua
Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
topic_facet Article
description A Ser105Ala mutant of the lipase B from Candida antarctica enables ‘perhydrolase-only’ reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.
format Text
author Wang, Xu-Ping
Zhou, Peng-Fei
Li, Zhi-Gang
Yang, Bo
Hollmann, Frank
Wang, Yong-Hua
author_facet Wang, Xu-Ping
Zhou, Peng-Fei
Li, Zhi-Gang
Yang, Bo
Hollmann, Frank
Wang, Yong-Hua
author_sort Wang, Xu-Ping
title Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_short Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_full Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_fullStr Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_full_unstemmed Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
title_sort engineering a lipase b from candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity
publisher Nature Publishing Group
publishDate 2017
url http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357956/
http://www.ncbi.nlm.nih.gov/pubmed/28317884
https://doi.org/10.1038/srep44599
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357956/
http://www.ncbi.nlm.nih.gov/pubmed/28317884
http://dx.doi.org/10.1038/srep44599
op_rights Copyright © 2017, The Author(s)
http://creativecommons.org/licenses/by/4.0/
This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
op_rightsnorm CC-BY
op_doi https://doi.org/10.1038/srep44599
container_title Scientific Reports
container_volume 7
container_issue 1
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