Engineering a lipase B from Candida antactica with efficient perhydrolysis performance by eliminating its hydrolase activity

A Ser105Ala mutant of the lipase B from Candida antarctica enables ‘perhydrolase-only’ reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially elimi...

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Bibliographic Details
Published in:Scientific Reports
Main Authors: Wang, Xu-Ping, Zhou, Peng-Fei, Li, Zhi-Gang, Yang, Bo, Hollmann, Frank, Wang, Yong-Hua
Format: Text
Language:English
Published: Nature Publishing Group 2017
Subjects:
Article
Antarc*
Antarctica
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357956/
http://www.ncbi.nlm.nih.gov/pubmed/28317884
https://doi.org/10.1038/srep44599
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Summary:A Ser105Ala mutant of the lipase B from Candida antarctica enables ‘perhydrolase-only’ reactions. At the example of the chemoenzymatic Baeyer-Villiger oxidation of cyclohexanone, we demonstrate that with this mutant selective oxidation can be achieved in deep eutectic solvent while essentially eliminating the undesired hydrolysis reaction of the product.

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