Combining Catalyzed Reporter Deposition-Fluorescence In Situ Hybridization and Microautoradiography To Detect Substrate Utilization by Bacteria and Archaea in the Deep Ocean

The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with micro...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Teira, Eva, Reinthaler, Thomas, Pernthaler, Annelie, Pernthaler, Jakob, Herndl, Gerhard J.
Format: Text
Language:English
Published: American Society for Microbiology 2004
Subjects:
Methods
North Atlantic
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC444763
http://www.ncbi.nlm.nih.gov/pubmed/15240332
https://doi.org/10.1128/AEM.70.7.4411-4414.2004
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Summary:The recently developed CARD-FISH protocol was refined for the detection of marine Archaea by replacing the lysozyme permeabilization treatment with proteinase K. This modification resulted in about twofold-higher detection rates for Archaea in deep waters. Using this method in combination with microautoradiography, we found that Archaea are more abundant than Bacteria (42% versus 32% of 4′,6′-diamidino-2-phenylindole counts) in the deep waters of the North Atlantic and that a larger fraction of Archaea than of Bacteria takes up l-aspartic acid (19% versus 10%).

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