Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua
Chloroquine (CQ) is used as a first-line therapy for the treatment of Plasmodium falciparum malaria in Nicaragua. We investigated the prevalence of molecular markers associated with CQ and sulfadoxine-pyrimethamine (SP) resistance in P. falciparum isolates obtained from the North Atlantic Autonomous...
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ftpubmed:oai:pubmedcentral.nih.gov:4015575 2023-05-15T17:32:44+02:00 Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua Sridaran, Sankar Rodriguez, Betzabe Mercedes Soto, Aida Macedo De Oliveira, Alexandre Udhayakumar, Venkatachalam 2014-05-07 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015575/ http://www.ncbi.nlm.nih.gov/pubmed/24615126 https://doi.org/10.4269/ajtmh.13-0214 en eng The American Society of Tropical Medicine and Hygiene http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015575/ http://www.ncbi.nlm.nih.gov/pubmed/24615126 http://dx.doi.org/10.4269/ajtmh.13-0214 ©The American Society of Tropical Medicine and Hygiene Articles Text 2014 ftpubmed https://doi.org/10.4269/ajtmh.13-0214 2015-05-09T23:57:12Z Chloroquine (CQ) is used as a first-line therapy for the treatment of Plasmodium falciparum malaria in Nicaragua. We investigated the prevalence of molecular markers associated with CQ and sulfadoxine-pyrimethamine (SP) resistance in P. falciparum isolates obtained from the North Atlantic Autonomous Region of Nicaragua. Blood spots for this study were made available from a CQ and SP drug efficacy trial conducted in 2005 and also from a surveillance study performed in 2011. Polymorphisms in P. falciparum CQ resistance transporter, dihydrofolate reductase, and dihydropteroate synthase gene loci that are associated with resistance to CQ, pyrimethamine, and sulfadoxine, respectively, were detected by DNA sequencing. In the 2005 dataset, only 2 of 53 isolates had a CQ resistance allele (CVIET), 2 of 52 had a pyrimethamine resistance allele, and 1 of 49 had a sulfadoxine resistance allele. In the 2011 dataset, none of 45 isolates analyzed had CQ or SP resistance alleles. Text North Atlantic PubMed Central (PMC) The American Journal of Tropical Medicine and Hygiene 90 5 840 845 |
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Articles Sridaran, Sankar Rodriguez, Betzabe Mercedes Soto, Aida Macedo De Oliveira, Alexandre Udhayakumar, Venkatachalam Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua |
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Chloroquine (CQ) is used as a first-line therapy for the treatment of Plasmodium falciparum malaria in Nicaragua. We investigated the prevalence of molecular markers associated with CQ and sulfadoxine-pyrimethamine (SP) resistance in P. falciparum isolates obtained from the North Atlantic Autonomous Region of Nicaragua. Blood spots for this study were made available from a CQ and SP drug efficacy trial conducted in 2005 and also from a surveillance study performed in 2011. Polymorphisms in P. falciparum CQ resistance transporter, dihydrofolate reductase, and dihydropteroate synthase gene loci that are associated with resistance to CQ, pyrimethamine, and sulfadoxine, respectively, were detected by DNA sequencing. In the 2005 dataset, only 2 of 53 isolates had a CQ resistance allele (CVIET), 2 of 52 had a pyrimethamine resistance allele, and 1 of 49 had a sulfadoxine resistance allele. In the 2011 dataset, none of 45 isolates analyzed had CQ or SP resistance alleles. |
format |
Text |
author |
Sridaran, Sankar Rodriguez, Betzabe Mercedes Soto, Aida Macedo De Oliveira, Alexandre Udhayakumar, Venkatachalam |
author_facet |
Sridaran, Sankar Rodriguez, Betzabe Mercedes Soto, Aida Macedo De Oliveira, Alexandre Udhayakumar, Venkatachalam |
author_sort |
Sridaran, Sankar |
title |
Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua |
title_short |
Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua |
title_full |
Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua |
title_fullStr |
Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua |
title_full_unstemmed |
Molecular Analysis of Chloroquine and Sulfadoxine-Pyrimethamine Resistance-Associated Alleles in Plasmodium falciparum Isolates from Nicaragua |
title_sort |
molecular analysis of chloroquine and sulfadoxine-pyrimethamine resistance-associated alleles in plasmodium falciparum isolates from nicaragua |
publisher |
The American Society of Tropical Medicine and Hygiene |
publishDate |
2014 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015575/ http://www.ncbi.nlm.nih.gov/pubmed/24615126 https://doi.org/10.4269/ajtmh.13-0214 |
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North Atlantic |
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North Atlantic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015575/ http://www.ncbi.nlm.nih.gov/pubmed/24615126 http://dx.doi.org/10.4269/ajtmh.13-0214 |
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©The American Society of Tropical Medicine and Hygiene |
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https://doi.org/10.4269/ajtmh.13-0214 |
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The American Journal of Tropical Medicine and Hygiene |
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90 |
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5 |
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840 |
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845 |
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1766130998876045312 |