Characterization of a pigment-dispersing hormone in eyestalks of the fiddler crab Uca pugilator

A pigment-dispersing hormone (PDH) from eyestalks of the fiddler crab Uca pugilator has been purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase liquid chromatography. Based on automated gas-phase sequencing and subsequent identification of carboxyl-...

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Bibliographic Details
Main Authors: Rao, K. Ranga, Riehm, John P., Zahnow, Cynthia A., Kleinholz, Lewis H., Tarr, George E., Johnson, Linda, Norton, Sheila, Landau, Matthew, Semmes, Oliver J., Sattelberg, R. Mark, Jorenby, William H., Hintz, Mary F.
Format: Text
Language:English
Published: 1985
Subjects:
Biological Sciences: Biochemistry
Pandalus borealis
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC390559
http://www.ncbi.nlm.nih.gov/pubmed/16593589
Description
Summary:A pigment-dispersing hormone (PDH) from eyestalks of the fiddler crab Uca pugilator has been purified by gel filtration, ion-exchange chromatography, partition chromatography, and reversed-phase liquid chromatography. Based on automated gas-phase sequencing and subsequent identification of carboxyl-terminal amide, we have assigned the primary structure of this peptide as Asn-Ser-Glu-Leu-Ile-Asn-Ser-Ile-Leu-Gly-Leu-Pro-Lys-Val-Met-Asn-Asp-Ala-NH 2. We have confirmed the sequence by synthesizing this peptide and demonstrating that the synthetic PDH and the native PDH display identical chromatographic behavior and biological activity. This hormone is a member of a family of invertebrate neuropeptides that includes a light-adapting/pigment-dispersing octadecapeptide hormone from the prawn Pandalus borealis. In assays for melanophore pigment dispersion in destalked fiddler crabs, Uca PDH was 21-fold more potent than Pandalus PDH. These two hormones share a hexapeptide core sequence (residues 5-10: -Ile-Asn-Ser-Ile-Leu-Gly-) as well as the amino- and carboxyl-terminal residues but differ at positions 3, 4, 11, 13, 16, and 17. These results point to speciesrelated or group-specific structural differences among crustacean PDHs.

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