Plasmid diversity in arctic strains of Psychrobacter spp.

Six strains of Psychrobacter spp. isolated from guano of little auks collected on Spitsbergen island (Arctic) carried nine plasmids that were fully sequenced. These replicons (ranging in size from 2917 to 14924 bp) contained either repA (ColE2-type) or repB (iteron-type) replication systems of a rel...

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Bibliographic Details
Published in:Extremophiles
Main Authors: Dziewit, Lukasz, Cegielski, Adrian, Romaniuk, Krzysztof, Uhrynowski, Witold, Szych, Antoni, Niesiobedzki, Pawel, Zmuda-Baranowska, Magdalena J., Zdanowski, Marek K., Bartosik, Dariusz
Format: Text
Language:English
Published: Springer Japan 2013
Subjects:
Original Paper
Arctic
Guano
Spitsbergen
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3632715
http://www.ncbi.nlm.nih.gov/pubmed/23479249
https://doi.org/10.1007/s00792-013-0521-0
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Summary:Six strains of Psychrobacter spp. isolated from guano of little auks collected on Spitsbergen island (Arctic) carried nine plasmids that were fully sequenced. These replicons (ranging in size from 2917 to 14924 bp) contained either repA (ColE2-type) or repB (iteron-type) replication systems of a relatively narrow host range, limited to Psychrobacter spp. All but one of the plasmids carried predicted mobilization for conjugal transfer systems, encoding relaxases of the MOBQ, MOBV or MOBP families. The plasmids also contained diverse additional genetic load, including a type II restriction-modification system and a gene encoding a putative subunit C of alkyl hydroperoxide reductase (AhpC)—an antioxidant enzyme and major scavenger of reactive oxygen species. Detailed comparative sequence analyses, extended to all plasmids identified so far in psychrophilic bacteria, distinguished groups of the most ubiquitous replicons, which play a key role in horizontal gene transfer in cold environments.

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