Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli▿

Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was...

Full description

Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Narayanan, Niju, Chou, C. Perry
Format: Text
Language:English
Published: American Society for Microbiology (ASM) 2009
Subjects:
Enzymology and Protein Engineering
Antarc*
Antarctica
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725454
http://www.ncbi.nlm.nih.gov/pubmed/19542329
https://doi.org/10.1128/AEM.00740-09
Description
Summary:Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression.

Similar Items