Alleviation of Proteolytic Sensitivity To Enhance Recombinant Lipase Production in Escherichia coli▿
Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology (ASM)
2009
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Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2725454 http://www.ncbi.nlm.nih.gov/pubmed/19542329 https://doi.org/10.1128/AEM.00740-09 |
Summary: | Two amino acids, Leu149 and Val223, were identified as proteolytically sensitive when Pseudozyma antarctica lipase (PalB) was heterologously expressed in Escherichia coli. The functional expression was enhanced using the double mutant for cultivation. However, the recombinant protein production was still limited by PalB misfolding, which was resolved by DsbA coexpression. |
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