Identification of a Novel Alkaliphilic Esterase Active at Low Temperatures by Screening a Metagenomic Library from Antarctic Desert Soil▿ †

A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature r...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Heath, Caroline, Hu, Xiao Ping, Cary, S. Craig, Cowan, Donald
Format: Text
Language:English
Published: American Society for Microbiology (ASM) 2009
Subjects:
Environmental Microbiology
Antarctic
Antarc*
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2704825
http://www.ncbi.nlm.nih.gov/pubmed/19411411
https://doi.org/10.1128/AEM.02597-08
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Summary:A novel esterase was identified through functional screening of a metagenomic library in Escherichia coli obtained from Antarctic desert soil. The 297-amino-acid sequence had only low (<29%) similarity to a putative esterase from Burkholderia xenovorans. The enzyme was active over a temperature range of 7 to 54°C and at alkaline pH levels and is a potential candidate for industrial application.

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