Flavobacterium sp. Strain 4221 and Pedobacter sp. Strain 4236 β-1,3-Glucanases That Are Active at Low Temperatures ▿ †

Secretion of β-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of β-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hyd...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Rasmussen, Mikkel A., Madsen, Søren M., Stougaard, Peter, Johnsen, Mads G.
Format: Text
Language:English
Published: American Society for Microbiology (ASM) 2008
Subjects:
Enzymology and Protein Engineering
Arctic
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2583487
http://www.ncbi.nlm.nih.gov/pubmed/18806001
https://doi.org/10.1128/AEM.00681-08
Description
Summary:Secretion of β-1,3-glucanases by the arctic bacterial isolates 4221 and 4236, related to the genera Flavobacterium and Pedobacter, was discovered. Escherichia coli and Lactococcus lactis expression of β-1,3-glucanases Glc4221-1 and Glc4236-1 from the respective isolates was achieved. The enzymes hydrolyzed fungal cell walls and retained activity at low temperatures.

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