Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿
Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent \documentclass[10pt]{article} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \us...
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Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446576 http://www.ncbi.nlm.nih.gov/pubmed/18424535 https://doi.org/10.1128/AEM.02928-07 |
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ftpubmed:oai:pubmedcentral.nih.gov:2446576 2023-05-15T15:01:10+02:00 Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. 2008-06 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446576 http://www.ncbi.nlm.nih.gov/pubmed/18424535 https://doi.org/10.1128/AEM.02928-07 en eng American Society for Microbiology (ASM) http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446576 http://www.ncbi.nlm.nih.gov/pubmed/18424535 http://dx.doi.org/10.1128/AEM.02928-07 Copyright © 2008, American Society for Microbiology Enzymology and Protein Engineering Text 2008 ftpubmed https://doi.org/10.1128/AEM.02928-07 2013-09-02T01:17:09Z Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent \documentclass[10pt]{article} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{pmc} \usepackage[Euler]{upgreek} \pagestyle{empty} \oddsidemargin -1.0in \begin{document} \begin{equation*}k_{cat}\end{equation*}\end{document} for biphenyl of 1.1 ± 0.1 s−1 (mean ± standard deviation) at 4°C. In contrast, BPDOLB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDOCam-1 oxygenase was less than half that of the BPDOLB400 oxygenase. Nevertheless, BPDOCam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. Text Arctic PubMed Central (PMC) Arctic Applied and Environmental Microbiology 74 12 3908 3911 |
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English |
topic |
Enzymology and Protein Engineering |
spellingShingle |
Enzymology and Protein Engineering Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ |
topic_facet |
Enzymology and Protein Engineering |
description |
Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent \documentclass[10pt]{article} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{pmc} \usepackage[Euler]{upgreek} \pagestyle{empty} \oddsidemargin -1.0in \begin{document} \begin{equation*}k_{cat}\end{equation*}\end{document} for biphenyl of 1.1 ± 0.1 s−1 (mean ± standard deviation) at 4°C. In contrast, BPDOLB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDOCam-1 oxygenase was less than half that of the BPDOLB400 oxygenase. Nevertheless, BPDOCam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. |
format |
Text |
author |
Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. |
author_facet |
Master, Emma R. Agar, Nathalie Y. R. Gómez-Gil, Leticia Powlowski, Justin B. Mohn, William W. Eltis, Lindsay D. |
author_sort |
Master, Emma R. |
title |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ |
title_short |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ |
title_full |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ |
title_fullStr |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ |
title_full_unstemmed |
Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿ |
title_sort |
biphenyl dioxygenase from an arctic isolate is not cold adapted▿ |
publisher |
American Society for Microbiology (ASM) |
publishDate |
2008 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446576 http://www.ncbi.nlm.nih.gov/pubmed/18424535 https://doi.org/10.1128/AEM.02928-07 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446576 http://www.ncbi.nlm.nih.gov/pubmed/18424535 http://dx.doi.org/10.1128/AEM.02928-07 |
op_rights |
Copyright © 2008, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.02928-07 |
container_title |
Applied and Environmental Microbiology |
container_volume |
74 |
container_issue |
12 |
container_start_page |
3908 |
op_container_end_page |
3911 |
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1766333204987379712 |