Biphenyl Dioxygenase from an Arctic Isolate Is Not Cold Adapted▿
Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent \documentclass[10pt]{article} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \us...
Published in: | Applied and Environmental Microbiology |
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Main Authors: | , , , , , |
Format: | Text |
Language: | English |
Published: |
American Society for Microbiology (ASM)
2008
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Subjects: | |
Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2446576 http://www.ncbi.nlm.nih.gov/pubmed/18424535 https://doi.org/10.1128/AEM.02928-07 |
Summary: | Biphenyl dioxygenase from the psychrotolerant bacterium Pseudomonas sp. strain Cam-1 (BPDOCam-1) was purified and found to have an apparent \documentclass[10pt]{article} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{pmc} \usepackage[Euler]{upgreek} \pagestyle{empty} \oddsidemargin -1.0in \begin{document} \begin{equation*}k_{cat}\end{equation*}\end{document} for biphenyl of 1.1 ± 0.1 s−1 (mean ± standard deviation) at 4°C. In contrast, BPDOLB400 from the mesophile Burkholderia xenovorans LB400 had no detectable activity at this temperature. At 57°C, the half-life of the BPDOCam-1 oxygenase was less than half that of the BPDOLB400 oxygenase. Nevertheless, BPDOCam-1 appears to be a typical Pseudomonas pseudoalcaligenes KF707-type dioxygenase. |
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