Elevated temperature method for recovery of Vibrio cholerae from oysters (Crassostrea gigas).

Of 222 Vibrio cholerae isolates from diverse clinical and environmental sources, 219 produced visible growth in alkaline peptone broth when incubated overnight at 42 degrees C. In field trials conducted to compare enrichment at incubation temperatures of 42 and 35 degrees C, significantly higher rat...

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Bibliographic Details
Main Authors: DePaola, A, Kaysner, C A, McPhearson, R M
Format: Text
Language:English
Published: 1987
Subjects:
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC203830
http://www.ncbi.nlm.nih.gov/pubmed/3606095
Description
Summary:Of 222 Vibrio cholerae isolates from diverse clinical and environmental sources, 219 produced visible growth in alkaline peptone broth when incubated overnight at 42 degrees C. In field trials conducted to compare enrichment at incubation temperatures of 42 and 35 degrees C, significantly higher rates of isolation (P less than 0.05) and recovery (P less than 0.01) of V. cholerae from oysters were observed at 42 degrees C.