Reaction of myoglobin with 3,3-tetramethyleneglutaric anhydride
1. The extent of reaction of the protein with 3,3-tetramethyleneglutaric anhydride was measured by determining the loss of lysine and the equivalent appearance of 3,3-tetramethyleneglutarimidolysine in the hydrolysate at various time-intervals. Of the 19 lysine residues in sperm-whale myoglobin, thr...
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| Format: | Text |
| Language: | English |
| Published: |
1967
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| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1270270 http://www.ncbi.nlm.nih.gov/pubmed/6029606 |
| Summary: | 1. The extent of reaction of the protein with 3,3-tetramethyleneglutaric anhydride was measured by determining the loss of lysine and the equivalent appearance of 3,3-tetramethyleneglutarimidolysine in the hydrolysate at various time-intervals. Of the 19 lysine residues in sperm-whale myoglobin, three did not react with 3,3-tetramethyleneglutaric anhydride. Also, the N-terminal valine did not react. 2. Slight spectral changes were observed on modification of myoglobin. 3. Electrophoretic changes were great since the derivative was negative throughout the range pH6–8·8. 4. The derivative did not react with antisera to native sperm-whale myoglobin. |
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