A versatile nuclei extraction protocol for single nucleus sequencing in non-model species–Optimization in various Atlantic salmon tissues

The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present...

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Bibliographic Details
Published in:PLOS ONE
Main Authors: Ruiz Daniels, Rose, Taylor, Richard S., Dobie, Ross, Salisbury, Sarah, Furniss, James J., Clark, Emily, Macqueen, Daniel J., Robledo, Diego
Format: Text
Language:English
Published: Public Library of Science 2023
Subjects:
Lab Protocol
Atlantic salmon
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484441/
https://doi.org/10.1371/journal.pone.0285020
Description
Summary:The use of single cell sequencing technologies has exploded over recent years, and is now commonly used in many non-model species. Sequencing nuclei instead of whole cells has become increasingly popular, as it does not require the processing of samples immediately after collection. Here we present a highly effective nucleus isolation protocol that outperforms previously available method in challenging samples in a non-model specie. This protocol can be successfully applied to extract nuclei from a variety of tissues and species.

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