Characterization and in vitro calcium release of the novel calcium-loaded complexes using Antarctic krill protein and pectin: Effect of different blending sequences.

• Published in: Food Research International
• Main Authors: Chen, Lei, Lin, Songyi, He, Xueqing, Ye, Jiaqi, Huang, Yihan, Sun, Na
• Format: Article in Journal/Newspaper
• Language: English
• Published: Elsevier Science 2024
• Subjects: Antarctic krill protein; Calcium delivery system; Calcium sustained-release; Pectin; Self-assembly sequence; Antarc*; Antarctic; Antarctic Krill
• Online Access: https://doi.org/10.1016/j.foodres.2024.114589; https://pubmed.ncbi.nlm.nih.gov/38945608

Food-grade biopolymer-based complexes are of particular interest in the field of biologic ingredient delivery owing to unique controlled-release properties. Herein, three calcium-loaded complexes using Antarctic krill protein (P) and pectin (HMP) with different blending sequences were designed, named P + Ca + HMP, P + HMP + Ca and HMP + Ca + P, respectively. The calcium-loaded capacity, structural properties, and in vitro gastrointestinal calcium release of the complexes were investigated. The results demonstrated that the calcium binding rate and content of the P + Ca + HMP complex were the highest, reaching to 90.3 % and 39.0 mg/g, respectively. Particularly, the P + Ca + HMP complex exhibited a more stable fruit tree-like structure. Furthermore, the structural analysis confirmed that the primary interaction forces involved hydrogen bond, electrostatic, hydrophobic and ionic bond interaction. Ultimately, the P + Ca + HMP complex demonstrated superior calcium delivery. In conclusion, a novel calcium delivery system was successfully developed based on optimized the self-assembly sequence, which held significant importance in promoting the high-value utilization of Antarctic krill protein and enhancing the in vitro bioaccessibility of calcium.