Genomics- and Transcriptomics-Guided Discovery of Clavatols from Arctic Fungi

Clavatols exhibit a wide range of biological activities due to their diverse structures. A genome mining strategy identified an A5cla cluster from Penicillium sp. MYA5, derived from the Arctic plant Dryas octopetala, is responsible for clavatol biosynthesis. Seven clavatols, including one new clavat...

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Bibliographic Details
Published in:Marine Drugs
Main Authors: Sun, Yuan-Yuan, Hu, Bo, Yu, Hao-Bing, Zhou, Jing, Meng, Xian-Chao, Ning, Zhe, Ding, Jin-Feng, Cui, Ming-Hui, Liu, Xiao-Yu
Format: Article in Journal/Newspaper
Language:English
Published: MDPI 2024
Subjects:
Penicillium sp.
clavatols
genomics
secondary metabolite
transcriptomics
Dryas octopetala
Online Access:https://doi.org/10.3390/md22060236
https://pubmed.ncbi.nlm.nih.gov/38921547
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11205228/
Description
Summary:Clavatols exhibit a wide range of biological activities due to their diverse structures. A genome mining strategy identified an A5cla cluster from Penicillium sp. MYA5, derived from the Arctic plant Dryas octopetala, is responsible for clavatol biosynthesis. Seven clavatols, including one new clavatol derivate named penicophenone F (1) and six known clavatols (2-7), were isolated from Penicillium sp. MYA5 using a transcriptome mining strategy. These structures were elucidated by comprehensive spectroscopic analysis. Antibacterial, aldose reductase inhibition, and siderophore-producing ability assays were conducted on compounds 1-7. Compounds 1 and 2 demonstrated inhibitory effects on the ALR2 enzyme with inhibition rates of 75.3% and 71.6% at a concentration of 10 μM, respectively. Compound 6 exhibited antibacterial activity against Staphylococcus aureus and Escherichia coli with MIC values of 4.0 μg/mL and 4.0 μg/mL, respectively. Additionally, compounds 1, 5, and 6 also showed potential iron-binding ability.

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