Palaeogenomic analysis of black rat (Rattus rattus) reveals multiple European introductions associated with human economic history

• Main Authors: Yu, H., Jamieson, A., Hulme-Beaman, A., Conroy, C., Knight, B., Speller, C., Al-Jarah, H., Eager, H., Trinks, A., Adikari, G., Baron, H., Böhlendorf-Arslan, B., Bohingamuwa, W., Crowther, A., Cucchi, T., Esser, K., Fleisher, J., Gidney, L., Gladilina, E., Gol’din, P., Goodman, S., Hamilton-Dyer, S., Helm, R., Hillman, C., Kallala, N., Kivikero, H., Kovács, Z., Karl Kunst, G., Kyselý, R., Linderholm, A., Maraoui-Telmini, B., Morales-Muñiz, A., Nabais, M., O’Connor, T., Oueslati, T., Morales, Q., M., E., Pasda, K., Perera, J., Perera, N., Radbauer, S., Ramon, J., Rannamäe, E., Sanmartí Grego, J., Treasure, E., Valenzuela-Lamas, S., van der Jagt, I., Van Neer, W., Vigne, J., Walker, T., Wynne-Jones, S., Zeiler, J., Dobney, K., Boivin, N., Searle, J., Krause-Kyora, B., Krause, J., Larson, G., Orton, D.
• Format: Report
• Language: English
• Published: 2022
• Subjects: Rattus rattus
• Online Access: http://hdl.handle.net/21.11116/0000-0009-52B8-B; http://hdl.handle.net/21.11116/0000-000A-7B7F-F; http://hdl.handle.net/21.11116/0000-000A-7B80-B

The distribution of the black rat (Rattus rattus) has been heavily influenced by its association with humans. The dispersal history of this non-native commensal rodent across Europe, however, remains poorly understood, and different introductions may have occurred during the Roman and medieval periods. Here, in order to reconstruct the population history of European black rats, we generated a de novo genome assembly of the black rat, 67 ancient black rat mitogenomes and 36 ancient nuclear genomes from sites spanning the 1st-17th centuries CE in Europe and North Africa. Analyses of mitochondrial DNA confirm that black rats were introduced into the Mediterranean and Europe from Southwest Asia. Genomic analyses of the ancient rats reveal a population turnover in temperate Europe between the 6th and 10th centuries CE, coincident with an archaeologically attested decline in the black rat population. The near disappearance and re-emergence of black rats in Europe may have been the result of the breakdown of the Roman Empire, the First Plague Pandemic, and/or post-Roman climatic cooling.Competing Interest StatementThe authors have declared no competing interest. - Results and Discussion -- The demographic history of Rattus rattus and its closely related species -- A global phylogeography of the black rat based on mitochondrial DNA -- Ancient genomes reveal the relationships of European black rats over space and time - Discussion - Methods