Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)

Table of Contents Affirmation . i Table of Contents . ii Index of Figures.iv Index of Tables .iv Index of Abbreviations . v 1 Introduction . 1 1.1 PRDM9 . 1 1.1.1 PRDM9 in Mus musculus . 2 1.1.2 PRDM9 in Humans . 4 1.1.3 PRDM9 in non-human Primates . 4 1.1.4 PRDM9 in Ruminants . 5 1.1.5 PRDM9 in Equ...

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Bibliographic Details
Main Author: Kovalchuk, E.
Format: Master Thesis
Language:English
Published: Christian-Albrechts-Universität zu Kiel 2018
Subjects:
Online Access:http://hdl.handle.net/21.11116/0000-0002-FCDE-9
http://hdl.handle.net/21.11116/0000-0003-C7A5-2
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spelling ftpubman:oai:pure.mpg.de:item_3027052 2023-08-27T04:08:36+02:00 Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera) Kovalchuk, E. 2018-03-25 application/pdf http://hdl.handle.net/21.11116/0000-0002-FCDE-9 http://hdl.handle.net/21.11116/0000-0003-C7A5-2 eng eng Christian-Albrechts-Universität zu Kiel http://hdl.handle.net/21.11116/0000-0002-FCDE-9 http://hdl.handle.net/21.11116/0000-0003-C7A5-2 info:eu-repo/semantics/closedAccess info:eu-repo/semantics/masterThesis 2018 ftpubman 2023-08-02T01:57:51Z Table of Contents Affirmation . i Table of Contents . ii Index of Figures.iv Index of Tables .iv Index of Abbreviations . v 1 Introduction . 1 1.1 PRDM9 . 1 1.1.1 PRDM9 in Mus musculus . 2 1.1.2 PRDM9 in Humans . 4 1.1.3 PRDM9 in non-human Primates . 4 1.1.4 PRDM9 in Ruminants . 5 1.1.5 PRDM9 in Equidae . 6 1.2 Balaenoptera Acutorostrata . 7 1.3 Objective . 10 2 Materials and Methods . 11 2.1 B.acutorostrata DNA Samples . 11 2.2 STR genotyping . 12 2.3 Mitochondrial DLoop Sequencing . 13 2.4 PRDM9-ZNF Typing . 14 3 Results . 15 3.1 mtD-Loop Sequencing . 15 3.2 Microsatellite Analysis . 17 3.3 PRDM9 Zinc Finger PCR . 19 3.4 PRDM9 Zinc Finger Typing . 20 3.5 Characterising the PRDM9 ZNF Array . 21 3.6 In silico C2H2 ZNF binding predictions . 22 4 Discussion . 24 5 References . 31 6 Appendix . 37 6.1 Sequences of the used primers. 37 Master Thesis Balaenoptera acutorostrata Max Planck Society: MPG.PuRe
institution Open Polar
collection Max Planck Society: MPG.PuRe
op_collection_id ftpubman
language English
description Table of Contents Affirmation . i Table of Contents . ii Index of Figures.iv Index of Tables .iv Index of Abbreviations . v 1 Introduction . 1 1.1 PRDM9 . 1 1.1.1 PRDM9 in Mus musculus . 2 1.1.2 PRDM9 in Humans . 4 1.1.3 PRDM9 in non-human Primates . 4 1.1.4 PRDM9 in Ruminants . 5 1.1.5 PRDM9 in Equidae . 6 1.2 Balaenoptera Acutorostrata . 7 1.3 Objective . 10 2 Materials and Methods . 11 2.1 B.acutorostrata DNA Samples . 11 2.2 STR genotyping . 12 2.3 Mitochondrial DLoop Sequencing . 13 2.4 PRDM9-ZNF Typing . 14 3 Results . 15 3.1 mtD-Loop Sequencing . 15 3.2 Microsatellite Analysis . 17 3.3 PRDM9 Zinc Finger PCR . 19 3.4 PRDM9 Zinc Finger Typing . 20 3.5 Characterising the PRDM9 ZNF Array . 21 3.6 In silico C2H2 ZNF binding predictions . 22 4 Discussion . 24 5 References . 31 6 Appendix . 37 6.1 Sequences of the used primers. 37
format Master Thesis
author Kovalchuk, E.
spellingShingle Kovalchuk, E.
Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
author_facet Kovalchuk, E.
author_sort Kovalchuk, E.
title Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
title_short Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
title_full Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
title_fullStr Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
title_full_unstemmed Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
title_sort unravelling the zinc finger diversity of recombination regulator prdm9 in minke whales (balaenoptera)
publisher Christian-Albrechts-Universität zu Kiel
publishDate 2018
url http://hdl.handle.net/21.11116/0000-0002-FCDE-9
http://hdl.handle.net/21.11116/0000-0003-C7A5-2
genre Balaenoptera acutorostrata
genre_facet Balaenoptera acutorostrata
op_relation http://hdl.handle.net/21.11116/0000-0002-FCDE-9
http://hdl.handle.net/21.11116/0000-0003-C7A5-2
op_rights info:eu-repo/semantics/closedAccess
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