Unravelling the zinc finger diversity of recombination regulator PRDM9 in minke whales (Balaenoptera)
Table of Contents Affirmation . i Table of Contents . ii Index of Figures.iv Index of Tables .iv Index of Abbreviations . v 1 Introduction . 1 1.1 PRDM9 . 1 1.1.1 PRDM9 in Mus musculus . 2 1.1.2 PRDM9 in Humans . 4 1.1.3 PRDM9 in non-human Primates . 4 1.1.4 PRDM9 in Ruminants . 5 1.1.5 PRDM9 in Equ...
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| Format: | Master Thesis |
| Language: | English |
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Christian-Albrechts-Universität zu Kiel
2018
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| Online Access: | http://hdl.handle.net/21.11116/0000-0002-FCDE-9 http://hdl.handle.net/21.11116/0000-0003-C7A5-2 |
| Summary: | Table of Contents Affirmation . i Table of Contents . ii Index of Figures.iv Index of Tables .iv Index of Abbreviations . v 1 Introduction . 1 1.1 PRDM9 . 1 1.1.1 PRDM9 in Mus musculus . 2 1.1.2 PRDM9 in Humans . 4 1.1.3 PRDM9 in non-human Primates . 4 1.1.4 PRDM9 in Ruminants . 5 1.1.5 PRDM9 in Equidae . 6 1.2 Balaenoptera Acutorostrata . 7 1.3 Objective . 10 2 Materials and Methods . 11 2.1 B.acutorostrata DNA Samples . 11 2.2 STR genotyping . 12 2.3 Mitochondrial DLoop Sequencing . 13 2.4 PRDM9-ZNF Typing . 14 3 Results . 15 3.1 mtD-Loop Sequencing . 15 3.2 Microsatellite Analysis . 17 3.3 PRDM9 Zinc Finger PCR . 19 3.4 PRDM9 Zinc Finger Typing . 20 3.5 Characterising the PRDM9 ZNF Array . 21 3.6 In silico C2H2 ZNF binding predictions . 22 4 Discussion . 24 5 References . 31 6 Appendix . 37 6.1 Sequences of the used primers. 37 |
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