Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification
Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to e...
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Online Access: | http://plymsea.ac.uk/id/eprint/6073/ https://doi.org/10.1111/1755-0998.12241 |
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ftplymouthml:oai:plymsea.ac.uk:6073 2023-05-15T15:01:18+02:00 Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification Smolina, I Kollias, S Poortvliet, M Nielsen, TG Lindeque, PK Castellani, C Møller, EF Blanco-Bercial, L Hoarau, G 2014-03-24 http://plymsea.ac.uk/id/eprint/6073/ https://doi.org/10.1111/1755-0998.12241 unknown Smolina, I; Kollias, S; Poortvliet, M; Nielsen, TG; Lindeque, PK; Castellani, C; Møller, EF; Blanco-Bercial, L; Hoarau, G. 2014 Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification. Molecular Ecology Resources. n/a-n/a. https://doi.org/10.1111/1755-0998.12241 <https://doi.org/10.1111/1755-0998.12241> Biology Ecology and Environment Marine Sciences Publication - Article PeerReviewed 2014 ftplymouthml https://doi.org/10.1111/1755-0998.12241 2022-09-13T05:48:32Z Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae. Article in Journal/Newspaper Arctic Calanus finmarchicus Calanus glacialis Calanus hyperboreus Zooplankton Copepods Plymouth Marine Science Electronic Archive (PlyMSEA - Plymouth Marine Laboratory, PML) Arctic Molecular Ecology Resources n/a n/a |
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Open Polar |
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Plymouth Marine Science Electronic Archive (PlyMSEA - Plymouth Marine Laboratory, PML) |
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ftplymouthml |
language |
unknown |
topic |
Biology Ecology and Environment Marine Sciences |
spellingShingle |
Biology Ecology and Environment Marine Sciences Smolina, I Kollias, S Poortvliet, M Nielsen, TG Lindeque, PK Castellani, C Møller, EF Blanco-Bercial, L Hoarau, G Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification |
topic_facet |
Biology Ecology and Environment Marine Sciences |
description |
Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae. |
format |
Article in Journal/Newspaper |
author |
Smolina, I Kollias, S Poortvliet, M Nielsen, TG Lindeque, PK Castellani, C Møller, EF Blanco-Bercial, L Hoarau, G |
author_facet |
Smolina, I Kollias, S Poortvliet, M Nielsen, TG Lindeque, PK Castellani, C Møller, EF Blanco-Bercial, L Hoarau, G |
author_sort |
Smolina, I |
title |
Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification |
title_short |
Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification |
title_full |
Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification |
title_fullStr |
Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification |
title_full_unstemmed |
Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification |
title_sort |
genome- and transcriptome-assisted development of nuclear insertion/deletion markers for calanus species (copepoda: calanoida) identification |
publishDate |
2014 |
url |
http://plymsea.ac.uk/id/eprint/6073/ https://doi.org/10.1111/1755-0998.12241 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic Calanus finmarchicus Calanus glacialis Calanus hyperboreus Zooplankton Copepods |
genre_facet |
Arctic Calanus finmarchicus Calanus glacialis Calanus hyperboreus Zooplankton Copepods |
op_relation |
Smolina, I; Kollias, S; Poortvliet, M; Nielsen, TG; Lindeque, PK; Castellani, C; Møller, EF; Blanco-Bercial, L; Hoarau, G. 2014 Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification. Molecular Ecology Resources. n/a-n/a. https://doi.org/10.1111/1755-0998.12241 <https://doi.org/10.1111/1755-0998.12241> |
op_doi |
https://doi.org/10.1111/1755-0998.12241 |
container_title |
Molecular Ecology Resources |
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n/a |
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_version_ |
1766333321021751296 |