Genome- and transcriptome-assisted development of nuclear insertion/deletion markers for Calanus species (Copepoda: Calanoida) identification

Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to e...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: Smolina, I, Kollias, S, Poortvliet, M, Nielsen, TG, Lindeque, PK, Castellani, C, Møller, EF, Blanco-Bercial, L, Hoarau, G
Format: Article in Journal/Newspaper
Language:unknown
Published: 2014
Subjects:
Biology
Ecology and Environment
Marine Sciences
Arctic
Calanus finmarchicus
Calanus glacialis
Calanus hyperboreus
Zooplankton
Copepods
Online Access:http://plymsea.ac.uk/id/eprint/6073/
https://doi.org/10.1111/1755-0998.12241
Description
Summary:Copepods of the genus Calanus are key zooplankton species in temperate to arctic marine ecosystems. Despite their ecological importance, species identification remains challenging. Furthermore, the recent report of hybrids among Calanus species highlights the need for diagnostic nuclear markers to efficiently identify parental species and hybrids. Using next-generation sequencing analysis of both the genome and transcriptome from two sibling species, Calanus finmarchicus and Calanus glacialis, we developed a panel of 12 nuclear insertion/deletion markers. All the markers showed species-specific amplicon length. Furthermore, most of the markers were successfully amplified in other Calanus species, allowing the molecular identification of Calanus helgolandicus, Calanus hyperboreus and Calanus marshallae.

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