Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid

• Published in: Talanta
• Main Authors: Cao, Qing, Quan, Li, He, Chiyang, Li, Na, Li, Kean, Liu, Feng
• Other Authors: Liu, F (reprint author), Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Chengfu Rd, Beijing 100871, Peoples R China., Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China., Anqing Normal Coll, Dept Chem, Anqing 246011, Peoples R China., Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Chengfu Rd, Beijing 100871, Peoples R China.
• Format: Journal/Newspaper
• Language: English
• Published: talanta 2008
• Subjects: Horseradish peroxidase; Enzyme activity; Partition; Ionic liquid; Aqueous biphasic system; ANTARCTICA LIPASE-B; 2-PHASE SYSTEM; SOLVENT-EXTRACTION; CROWN-ETHER; SEPARATION; WATER; SALT; SELECTIVITY; STABILITY; MEDIA; Antarc*; Antarctica
• Online Access: https://hdl.handle.net/20.500.11897/197785; https://doi.org/10.1016/j.talanta.2008.05.055

Enzyme activity and partition behavior in aqueous biphasic systems (ABSs) consisting of ionic liquid (IL) and salt (IL-ABSs) were investigated to increase our understanding of IL-ABSs and shed light on their application potential as enzyme extraction system. With horseradish peroxidase (HRP) as the model enzyme, several effects of alkylimidazolium chloride-K2HPO4 ABSs on activity and partition behavior of enzyme were studied including alkyl chain length of ILs and concentrations of each component. High lyotropic ILs (1-butyl-3-methylimidazolium and 1-ethyl-3-methylimidazolium chloride) and adequate water content (>40%) were both essential for the activity maintenance of HRP in IL-ABS. 1-Butyl-3-methylimidazolium chloride ([C(4)mim]Cl) was found to be an appropriate IL for phase forming and HRP activity retaining. After optimization of phase condition, about 80% HRP amount was distributed in the IL-rich upper phase, and greater than 90% enzyme activity was obtained. Moreover, compared with the commonly used polymer-based ABSs, this [C(4)mim]Cl-ABS has a much lower viscosity, which is very beneficial to the experimental operation. Therefore, the tested IL-ABS could be considered as a potential enzyme extraction system. (C) 2008 Elsevier B.V. All rights reserved. Chemistry, Analytical SCI(E) EI PubMed 30 ARTICLE 1 160-165 77