Partition of horseradish peroxidase with maintained activity in aqueous biphasic system based on ionic liquid

Enzyme activity and partition behavior in aqueous biphasic systems (ABSs) consisting of ionic liquid (IL) and salt (IL-ABSs) were investigated to increase our understanding of IL-ABSs and shed light on their application potential as enzyme extraction system. With horseradish peroxidase (HRP) as the...

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Bibliographic Details
Published in:Talanta
Main Authors: Cao, Qing, Quan, Li, He, Chiyang, Li, Na, Li, Kean, Liu, Feng
Other Authors: Liu, F (reprint author), Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Chengfu Rd, Beijing 100871, Peoples R China., Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Beijing 100871, Peoples R China., Anqing Normal Coll, Dept Chem, Anqing 246011, Peoples R China., Peking Univ, Coll Chem, Minist Educ,Beijing Natl Lab Mol Sci, Key Lab Bioorgan Chem & Mol Engn, Chengfu Rd, Beijing 100871, Peoples R China.
Format: Journal/Newspaper
Language:English
Published: talanta 2008
Subjects:
Horseradish peroxidase
Enzyme activity
Partition
Ionic liquid
Aqueous biphasic system
ANTARCTICA LIPASE-B
2-PHASE SYSTEM
SOLVENT-EXTRACTION
CROWN-ETHER
SEPARATION
WATER
SALT
SELECTIVITY
STABILITY
MEDIA
Antarc*
Antarctica
Online Access:https://hdl.handle.net/20.500.11897/197785
https://doi.org/10.1016/j.talanta.2008.05.055
Description
Summary:Enzyme activity and partition behavior in aqueous biphasic systems (ABSs) consisting of ionic liquid (IL) and salt (IL-ABSs) were investigated to increase our understanding of IL-ABSs and shed light on their application potential as enzyme extraction system. With horseradish peroxidase (HRP) as the model enzyme, several effects of alkylimidazolium chloride-K2HPO4 ABSs on activity and partition behavior of enzyme were studied including alkyl chain length of ILs and concentrations of each component. High lyotropic ILs (1-butyl-3-methylimidazolium and 1-ethyl-3-methylimidazolium chloride) and adequate water content (>40%) were both essential for the activity maintenance of HRP in IL-ABS. 1-Butyl-3-methylimidazolium chloride ([C(4)mim]Cl) was found to be an appropriate IL for phase forming and HRP activity retaining. After optimization of phase condition, about 80% HRP amount was distributed in the IL-rich upper phase, and greater than 90% enzyme activity was obtained. Moreover, compared with the commonly used polymer-based ABSs, this [C(4)mim]Cl-ABS has a much lower viscosity, which is very beneficial to the experimental operation. Therefore, the tested IL-ABS could be considered as a potential enzyme extraction system. (C) 2008 Elsevier B.V. All rights reserved. Chemistry, Analytical SCI(E) EI PubMed 30 ARTICLE 1 160-165 77

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