Temperature responses of different functional traits in Arctic phytoplankton

• Main Authors: Rehder, Linda, Rokitta, Sebastian D, Buschmann, Isabelle, Jasper, Levke, Hoppe, Clara Jule Marie, Rost, Björn
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: Absorption cross section of PSII photochemistry; Calculated; Carbon; organic; particulate; net production; per cell; particulate/Nitrogen; particulate ratio; Chlorophyll a/particulate organic carbon ratio; Chlorophyll a per cell; Daily cell counting; Beckmann coulter Counter; Diatom; Electron transport rate; absolute; Experiment; Fast repetition rate fluorometry (FRRF); Flash combustions technique; CN Analyzer; EuroVector EA 3000; Fluorometry; Generation; growth; Growth rate; ice algae; Laboratory; Light saturation; Light use efficiency; Location; Maximal electron transport rate; Medium; Nitrogen; Phaeocystis pouchetii; physiology; Phytoplankton; Quantum yield efficiency of photosystem II; Sample ID; Sampling date; Species
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.968670; https://doi.org/10.1594/PANGAEA.968670

This study was conducted in autumn/winter 2021/2022 at the laboratory of the Alfred-Wegener-Institute Bremerhaven. To assess temperature response patterns of various functional traits in Arctic phytoplankton species, we cultivated Thalassiosira hyalina, Phaeocystis pouchetii, Micromonas pusilla and Nitzschia frigida at a temperature gradient of 0°C to 14°C under saturating light of 100 µmol photons m-2 s-1 (T. hyalina, P. pouchetii, M. pusilla) or 30 µmol photons m-2 s-1 (N. frigida) and replete nutrient conditions (micronutrients following F/2; 100 µmol L-1 nitrate, 100 µmol L-1 silicate, 6 µmol L-1 phosphate) in dilute batch cultures. Culture bottles were aerated with air containing a pCO2 of 400 µatm ensure a stabilized carbonate chemistry and to avoid sedimentation. After an acclimation period of >7 generation or >10 days, respectively, various functional traits were obtained: specific growth rate, quotas of particulate organic carbon (POC) and nitrogen (PON) as well as chlorophyll a (Chl a), photo-physiology using fast repetition rate fluorometry. Furthermore, the following parameters were calculated from the obtained data: POC:PON, Chl a:POC, POC production rate, photosystem II quantum yield, absolute electron transport rates at in-situ irradiance and maximal irradiance, light use efficiency, light acclimation indices, lifetime of PSII reopening and absorption cross section.