Respiration rates of the cold-water coral Desmophyllum dianthus during an ex-situ experiment in the Azores

• Main Authors: Carreiro-Silva, Marina, Godinho, Antonio, Sire de Vilar, Anaïs, Arzeni, Beatriz, Edery, Gal-la, Caetano, Miguel, Raimundo, Joana, Martins, Ines
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: Aquaria ID; Atlantic; Azores; calcification; Coral; surface area; deep-sea coral; Desmophyllum_dianthus_Aquaria_Experiment; Desmophyllum_dianthus_Sampling_Site; ecophysiology; Experiment; Experiment duration; iAtlantic; ImageJ (Version 1.52a); Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; LLDEP; Longline deployment; metabolism; Oxygen Dipping Probe; DP-PSt3; Fibox 4; PreSens; Respiration rate; oxygen; Sample ID; Species; unique identification; unique identification (Semantic URI); unique identification (URI); Treatment: carbon dioxide; partial pressure; Treatment: food; Treatment: mining; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.966828; https://doi.org/10.1594/PANGAEA.966828

We conducted a medium-term (4 months) multiple stressor experiment with the cold-water coral Desmophyllum dianthus under future environmental conditions (IPCC RCP 8.5 scenarios for 2100), and sediment plumes generated during the potential extraction of seafloor massive sulphides. The experiment followed a two-step approach, where during the first 3 months, corals were exposed to four different treatments combining predicted scenarios of ocean acidification (pCO2/pH) and food availability. Two levels of pCO2 conditions were considered: natural habitat present day conditions (~500 µatm, 720 m depth) and IPCC RCP8.5 scenario (1000 µatm; IPCC, 2019), corresponding to pHT values of 7.93 and 7.66, respectively. In addition, two food availability regimes were recreated: high frequency of feeding (food delivered twice a day / 7 days a week) and low frequency of feeding (food delivered every other day). There were six replicate 13 L aquaria per treatment (4 D. dianthus per aquaria) at a temperature of 10.5 ± 0.1 ºC. During the fourth month of the experiment, suspended polymetallic sulphide particles generated during potential mining activities were added to half of the aquaria under the climate change scenarios at a concentration of 10 mg/l, making 8 treatments in a fully crossed experimental design for the 3 factors tested (OA, food, mining particles). Measurements of respiration were made at different times during the experiments: T0 (immediately before the start of the experiment), 2, 4, 9 and 14 weeks, using an oxygen meter Fibox4 with PSt3 sensors (PreSens, Germany). Coral respiration rates were normalized to the coral skeletal surface area.