Carbonate chemistry of an aquaria setup during a multi-stressor experiment with the cold-water coral Dendrophyllia cornigera

• Main Authors: Gutiérrez-Zárate, Cristina, Veiga, Alfredo, Gori, Andrea, Gerpe, Fabián, Domínguez, Leo, Rodríguez, Alberto, Arias, Noelia, Álvarez, Marta, Acerbi, Rubén, Vázquez, Lucía, Movilla, Juancho, Varela, Marta M, Alba-Salgueiro, Rodrigo, Valencia-Vila, Joaquín, Orejas, Covadonga
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: Alkalinity; total; Aragonite saturation state; Atlantic Ocean; Bicarbonate ion; Calcite saturation state; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate chemistry; Carbonate ion; Carbon dioxide; DATE/TIME; Dendrophyllia_Bycatch; Experimental treatment; Experiment duration; iAtlantic; Integrated Assessment of Atlantic Marine Ecosystems in Space and Time; Laboratory experiment; NE Atlantic; NW Spain; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); pH; Potentiometric titration; Replicate; Salinity; Species; unique identification (Semantic URI); unique identification (URI); Spectrophotometric; Treatment: temperature; Type of study; WTW Multi Set 350i multiparameter probe; Carbonic acid
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.966668; https://doi.org/10.1594/PANGAEA.966668

A 9-month aquarium experiment with the cold-water Dendrophyllia cornigera was conducted to investigate the single and combined effects of warming, acidification and deoxygenation on its ecophysiological response. The experiment took place at the Aquarium finisterrae (A Coruña, Spain) between 2022-05-06 and 2023-02-24. Treatment values for each parameter (current in situ vs. climate change) were: 12 °C and 15 °C (temperature); ~7.99 and 7.69 (pH); ~8.63 mg/L and 6.45 mg/L (dissolved oxygen concentration). A total of eight treatments (with 3 replicates each, 5 L aquaria) were set up. Measurements for pH and total alkalinity (TA) were performed on seawater samples from all the experimental aquaria every 1 – 2 months. Samples for pH were directly collected on cylindrical 10 cm cuvettes and analysed on daily basis. After being thermostated at 25ºC, samples were measured using a manual spectrophotometrical procedure with a Sigma Aldrich impure indicator (Clayton and Byrne, 1993). TA was measured following the double end point potentiometric technique by Pérez and Fraga (1987a) and Pérez et al. (2000). Measurements of Certified Reference Material (Dickson's lab, SIO) were performed in order to control the accuracy of the TA measurements. The uncertainty of TA and pH is about 3 μmol/kg and 0.005 pH units, respectively. In situ additional CO2 system variables were calculated using the R package seacarb (Gattuso et al. 2023) using in situ temperature, salinity, measured pH and TA, the dissociation constants of carbonic acid in seawater (K1 and K2) by Lueker et al. (2000), the equilibrium constant of hydrogen fluoride by Perez and Fraga (1987b), the concentration of total boron by Uppström (1974) formulation, and the stability constant of hydrogen sulphate by Dickson (1990). Mean values for the concentration of silicate and phosphate on the aquaria experiments were used in the CO2 calculations, respectively, mean (and standard) deviations as 2.1 (0.4) and 0.31 (0.03) μmol/kg.