Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea
This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed spo...
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.966335 https://doi.org/10.1594/PANGAEA.966335 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.966335 2024-05-19T07:31:53+00:00 Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea Leys, Sally P Federwisch, Luisa MEDIAN LATITUDE: -63.826788 * MEDIAN LONGITUDE: -58.016557 * SOUTH-BOUND LATITUDE: -64.057780 * WEST-BOUND LONGITUDE: -58.477560 * NORTH-BOUND LATITUDE: -63.615860 * EAST-BOUND LONGITUDE: -57.501430 * DATE/TIME START: 2018-03-05T14:18:00 * DATE/TIME END: 2018-03-07T17:54:00 * MINIMUM ELEVATION: -845.0 m * MAXIMUM ELEVATION: -429.0 m 2024 text/tab-separated-values, 360 data points https://doi.pangaea.de/10.1594/PANGAEA.966335 https://doi.org/10.1594/PANGAEA.966335 en eng PANGAEA https://doi.org/10.1594/PANGAEA.965868 https://doi.pangaea.de/10.1594/PANGAEA.966335 https://doi.org/10.1594/PANGAEA.966335 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Agassiz Trawl AGT Antarctic AWI_BPP Bentho-Pelagic Processes @ AWI Demospongiae Elevation of event Event label Hexactinellida Image Image number/name James Clark Ross JR17003a JR17003A_38 JR17003A_46 JR17003A_52 Latitude of event Longitude of event microbes microbiome microscopy Prince Gustav Channel Sample ID Sponges TEM Transmission electron microscope (TEM) transmission electron microscopy ultrastructure Weddell Sea Dataset 2024 ftpangaea https://doi.org/10.1594/PANGAEA.96633510.1594/PANGAEA.965868 2024-04-23T23:36:34Z This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale. Dataset Antarc* Antarctic Weddell Sea PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(-58.477560,-57.501430,-63.615860,-64.057780) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Agassiz Trawl AGT Antarctic AWI_BPP Bentho-Pelagic Processes @ AWI Demospongiae Elevation of event Event label Hexactinellida Image Image number/name James Clark Ross JR17003a JR17003A_38 JR17003A_46 JR17003A_52 Latitude of event Longitude of event microbes microbiome microscopy Prince Gustav Channel Sample ID Sponges TEM Transmission electron microscope (TEM) transmission electron microscopy ultrastructure Weddell Sea |
spellingShingle |
Agassiz Trawl AGT Antarctic AWI_BPP Bentho-Pelagic Processes @ AWI Demospongiae Elevation of event Event label Hexactinellida Image Image number/name James Clark Ross JR17003a JR17003A_38 JR17003A_46 JR17003A_52 Latitude of event Longitude of event microbes microbiome microscopy Prince Gustav Channel Sample ID Sponges TEM Transmission electron microscope (TEM) transmission electron microscopy ultrastructure Weddell Sea Leys, Sally P Federwisch, Luisa Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
topic_facet |
Agassiz Trawl AGT Antarctic AWI_BPP Bentho-Pelagic Processes @ AWI Demospongiae Elevation of event Event label Hexactinellida Image Image number/name James Clark Ross JR17003a JR17003A_38 JR17003A_46 JR17003A_52 Latitude of event Longitude of event microbes microbiome microscopy Prince Gustav Channel Sample ID Sponges TEM Transmission electron microscope (TEM) transmission electron microscopy ultrastructure Weddell Sea |
description |
This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale. |
format |
Dataset |
author |
Leys, Sally P Federwisch, Luisa |
author_facet |
Leys, Sally P Federwisch, Luisa |
author_sort |
Leys, Sally P |
title |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
title_short |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
title_full |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
title_fullStr |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
title_full_unstemmed |
Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea |
title_sort |
ultrastructure micrographs of antarctic deep-water sponges collected for microbiome study during expedition jr17003a in the western weddell sea |
publisher |
PANGAEA |
publishDate |
2024 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.966335 https://doi.org/10.1594/PANGAEA.966335 |
op_coverage |
MEDIAN LATITUDE: -63.826788 * MEDIAN LONGITUDE: -58.016557 * SOUTH-BOUND LATITUDE: -64.057780 * WEST-BOUND LONGITUDE: -58.477560 * NORTH-BOUND LATITUDE: -63.615860 * EAST-BOUND LONGITUDE: -57.501430 * DATE/TIME START: 2018-03-05T14:18:00 * DATE/TIME END: 2018-03-07T17:54:00 * MINIMUM ELEVATION: -845.0 m * MAXIMUM ELEVATION: -429.0 m |
long_lat |
ENVELOPE(-58.477560,-57.501430,-63.615860,-64.057780) |
genre |
Antarc* Antarctic Weddell Sea |
genre_facet |
Antarc* Antarctic Weddell Sea |
op_relation |
https://doi.org/10.1594/PANGAEA.965868 https://doi.pangaea.de/10.1594/PANGAEA.966335 https://doi.org/10.1594/PANGAEA.966335 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.96633510.1594/PANGAEA.965868 |
_version_ |
1799469766252953600 |