Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea

• Main Authors: Leys, Sally P, Federwisch, Luisa
• Format: Dataset
• Language: English
• Published: PANGAEA 2024
• Subjects: Agassiz Trawl; AGT; Antarctic; AWI_BPP; Bentho-Pelagic Processes @ AWI; Demospongiae; Elevation of event; Event label; Hexactinellida; Image; Image number/name; James Clark Ross; JR17003a; JR17003A_38; JR17003A_46; JR17003A_52; Latitude of event; Longitude of event; microbes; microbiome; microscopy; Prince Gustav Channel; Sample ID; Sponges; TEM; Transmission electron microscope (TEM); transmission electron microscopy; ultrastructure; Weddell Sea; Antarc*
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.966335; https://doi.org/10.1594/PANGAEA.966335

This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale.