Ultrastructure micrographs of Antarctic deep-water sponges collected for microbiome study during expedition JR17003a in the western Weddell Sea

This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed spo...

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Bibliographic Details
Main Authors: Leys, Sally P, Federwisch, Luisa
Format: Dataset
Language:English
Published: PANGAEA 2024
Subjects:
Agassiz Trawl
AGT
Antarctic
AWI_BPP
Bentho-Pelagic Processes @ AWI
Demospongiae
Elevation of event
Event label
Hexactinellida
Image
Image number/name
James Clark Ross
JR17003a
JR17003A_38
JR17003A_46
JR17003A_52
Latitude of event
Longitude of event
microbes
microbiome
microscopy
Prince Gustav Channel
Sample ID
Sponges
TEM
Transmission electron microscope (TEM)
transmission electron microscopy
ultrastructure
Weddell Sea
Antarc*
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.966335
https://doi.org/10.1594/PANGAEA.966335
Description
Summary:This image dataset provides ultrastructural micrographs of Antarctic deep-water sponges (Demospongiae and Hexactinellida) collected for study of microbial symbionts and histology during expedition JR17003a (RRS James Clark Ross, 2018) in Prince Gustav Channel, western Weddell Sea. Formalin-fixed sponge tissues were rinsed twice in PBS and postfixed in 1% osmium tetroxide in phosphate buffer for 6 hours at 4°C. Tissues were then rinsed twice in distilled water, dehydrated through to 100% ethanol and embedded in epoxy (TAAB 812). Ultrastructural sections of 60 nm were cut with a Leica Ultracut T microtome, stained in uranyl acetate and lead citrate, and then viewed and photographed with a Philips Morgagni transmission electron microscope equipped with a Gatan CCD camera. Image files are labeled with expedition, station and sample ID. Each image includes a scale.

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