Lipid content of liver and muscle tissue of juvenile turbot (Scophthalmus maximus) fed with emerging protein sources

This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to...

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Bibliographic Details
Main Authors: Hörterer, Christina, Lannig, Gisela, Buck, Bela Hieronymus
Format: Dataset
Language:English
Published: PANGAEA 2024
Subjects:
by-product
Experiment
glucose
glycogen
Identification
insect meal
Laboratory balance
Sartorius
Secura 5102-1S
Laboratory experiment
Lipid
Location
Method comment
Sample ID
Sampling date/time
Scophthalmus maximus
liver lipid
per dry mass
muscle lipid
Species
unique identification
unique identification (Semantic URI)
unique identification (URI)
Tank number
Time point
descriptive
Treatment
Type of study
Alfred Wegener Institute
Turbot
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.960775
https://doi.org/10.1594/PANGAEA.960775
Description
Summary:This study examined the growth response of juvenile turbot (Scophthalmus maximus) to diets with graded fishmeal (FM) replacement with plant, animal, and emerging protein sources (PLANT, PAP, and MIX) in comparison to a commercial-like diet (CTRL). The feeding experiment was carried out from April to July 2019 in the Centre for Aquaculture Research (ZAF) at the Alfred Wegener Institute for Polar and Marine research in Bremerhaven, Germany. The juvenile turbot (Scophthalmus maximus) were purchased from France Turbot (L'Épine, France) and acclimated to the recirculating aquaculture system (RAS) for 2 weeks prior to starting the 16 weeks experimental trial. To elucidate the effects of the protein sources and the level of FM replacement on the nutritional status of the fish lipid content of liver and muscle tissue were determined at the end of the experiment (t4). Muscle and liver samples (3 individual fish per tank; 15 fish per diet in total) were grinded under liquid nitrogen. Due to small volume, the individual samples of liver and muscle were pooled (n=5 tanks per diet) for the crude lipid content. Following the method of Folch et al. (1957) and Postel et al. (2000), the lipids in the muscle and liver tissue were extracted with 2:1 dichloromethanemethanol (v/v) and an aqueous solution of 0.88% potassium chloride (KCl) (w:v). Crude lipid content was determined gravimetrically to the nearest 0.001 g and calculated as the percentage of lipids of tissue wet weight.

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