Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates
Object:Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the perfor...
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Format: | Dataset |
Language: | English |
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PANGAEA
2023
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Online Access: | https://doi.pangaea.de/10.1594/PANGAEA.959731 https://doi.org/10.1594/PANGAEA.959731 |
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.959731 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlamys islandica Coast and continental shelf Exponential rate constant for recovery standard deviation Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Halftime of recovery Laboratory experiment Maximal surplus oxidative flux adenosine triphosphate per time Mollusca North Atlantic Number Number of claps OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pecten maximus pH Phosphate inorganic/phospho-L-arginine ratio Phospho-L-arginine |
spellingShingle |
Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlamys islandica Coast and continental shelf Exponential rate constant for recovery standard deviation Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Halftime of recovery Laboratory experiment Maximal surplus oxidative flux adenosine triphosphate per time Mollusca North Atlantic Number Number of claps OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pecten maximus pH Phosphate inorganic/phospho-L-arginine ratio Phospho-L-arginine Bock, Christian Wermter, Felizitas Charlotte Schalkhausser, Burgel Blicher, Martin E Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates |
topic_facet |
Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlamys islandica Coast and continental shelf Exponential rate constant for recovery standard deviation Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Halftime of recovery Laboratory experiment Maximal surplus oxidative flux adenosine triphosphate per time Mollusca North Atlantic Number Number of claps OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pecten maximus pH Phosphate inorganic/phospho-L-arginine ratio Phospho-L-arginine |
description |
Object:Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the performance of marine invertebrates. Materials & methods: Experiments were conducted following the recommendations for studies of muscle bioenergetics in vertebrates. Animals were long-term incubated under different environmental conditions: controls at 0 °C for C. islandica and 15 °C for P. maximus under ambient PCO2 of 0.039 kPa, a warm exposure with +5 °C (5 °C and 20 °C, respectively) under ambient PCO2 (OW group), and a combined exposure to warmed acidified conditions (5 °C and 20 °C, 0.112 kPa PCO2, OWA group). Scallops were placed in a 4.7 T MR animal scanner and the energetic status of the adductor muscle was determined under resting conditions using in vivo 31P-NMR spectroscopy. The surplus oxidative flux (Qmax) was quantified by recording the recovery of arginine phosphate (PLA) directly after moderate swimming exercise of the scallops. Results:Measurements led to reproducible results within each experimental group. Under projected future conditions resting PLA levels (PLArest) were reduced, indicating reduced energy reserves in warming exposed scallops per se. In comparison to vertebrate muscle tissue surplus Qmax of scallop muscle was about one order of magnitude lower. This can be explained by lower mitochondrial contents and capacities in invertebrate than vertebrate muscle tissue. Warm exposed scallops showed a slower recovery rate of PLA levels (kPLA) and a reduced surplus Qmax. Elevated PCO2 did not affected PLA recovery further. |
format |
Dataset |
author |
Bock, Christian Wermter, Felizitas Charlotte Schalkhausser, Burgel Blicher, Martin E Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K |
author_facet |
Bock, Christian Wermter, Felizitas Charlotte Schalkhausser, Burgel Blicher, Martin E Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K |
author_sort |
Bock, Christian |
title |
Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates |
title_short |
Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates |
title_full |
Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates |
title_fullStr |
Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates |
title_full_unstemmed |
Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates |
title_sort |
seawater carbonate chemistry and in vivo 31p-mrs of muscle bioenergetics in marine invertebrates |
publisher |
PANGAEA |
publishDate |
2023 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.959731 https://doi.org/10.1594/PANGAEA.959731 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic North Atlantic Ocean acidification |
genre_facet |
Arctic North Atlantic Ocean acidification |
op_relation |
Bock, Christian; Wermter, Felizitas Charlotte; Schalkhausser, Burgel; Blicher, Martin E; Pörtner, Hans-Otto; Lannig, Gisela; Sejr, Mikael K (2019): In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance. Magnetic Resonance Imaging, 61, 239-246, https://doi.org/10.1016/j.mri.2019.06.003 Bock, Christian; Wermter, Felizitas Charlotte; Schalkhausser, Burgel; Blicher, Martin E; Pörtner, Hans-Otto; Lannig, Gisela; Sejr, Mikael K (2020): In vivo 31P-MRS data of muscle bioenergetics of Chlamys islandica and Pecten maximus. PANGAEA, https://doi.org/10.1594/PANGAEA.915931 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.959731 https://doi.org/10.1594/PANGAEA.959731 |
op_rights |
CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.95973110.1016/j.mri.2019.06.00310.1594/PANGAEA.915931 |
_version_ |
1772812948780089344 |
spelling |
ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.959731 2023-07-30T04:02:13+02:00 Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates Bock, Christian Wermter, Felizitas Charlotte Schalkhausser, Burgel Blicher, Martin E Pörtner, Hans-Otto Lannig, Gisela Sejr, Mikael K 2023 text/tab-separated-values, 220 data points https://doi.pangaea.de/10.1594/PANGAEA.959731 https://doi.org/10.1594/PANGAEA.959731 en eng PANGAEA Bock, Christian; Wermter, Felizitas Charlotte; Schalkhausser, Burgel; Blicher, Martin E; Pörtner, Hans-Otto; Lannig, Gisela; Sejr, Mikael K (2019): In vivo 31P-MRS of muscle bioenergetics in marine invertebrates: Future ocean limits scallops' performance. Magnetic Resonance Imaging, 61, 239-246, https://doi.org/10.1016/j.mri.2019.06.003 Bock, Christian; Wermter, Felizitas Charlotte; Schalkhausser, Burgel; Blicher, Martin E; Pörtner, Hans-Otto; Lannig, Gisela; Sejr, Mikael K (2020): In vivo 31P-MRS data of muscle bioenergetics of Chlamys islandica and Pecten maximus. PANGAEA, https://doi.org/10.1594/PANGAEA.915931 Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2022): seacarb: seawater carbonate chemistry with R. R package version 3.3.1. https://cran.r-project.org/web/packages/seacarb/index.html https://doi.pangaea.de/10.1594/PANGAEA.959731 https://doi.org/10.1594/PANGAEA.959731 CC-BY-4.0: Creative Commons Attribution 4.0 International Access constraints: unrestricted info:eu-repo/semantics/openAccess Alkalinity total Animalia Aragonite saturation state Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Chlamys islandica Coast and continental shelf Exponential rate constant for recovery standard deviation Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Halftime of recovery Laboratory experiment Maximal surplus oxidative flux adenosine triphosphate per time Mollusca North Atlantic Number Number of claps OA-ICC Ocean Acidification International Coordination Centre Other studied parameter or process Partial pressure of carbon dioxide Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Pecten maximus pH Phosphate inorganic/phospho-L-arginine ratio Phospho-L-arginine Dataset 2023 ftpangaea https://doi.org/10.1594/PANGAEA.95973110.1016/j.mri.2019.06.00310.1594/PANGAEA.915931 2023-07-14T05:50:08Z Object:Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the performance of marine invertebrates. Materials & methods: Experiments were conducted following the recommendations for studies of muscle bioenergetics in vertebrates. Animals were long-term incubated under different environmental conditions: controls at 0 °C for C. islandica and 15 °C for P. maximus under ambient PCO2 of 0.039 kPa, a warm exposure with +5 °C (5 °C and 20 °C, respectively) under ambient PCO2 (OW group), and a combined exposure to warmed acidified conditions (5 °C and 20 °C, 0.112 kPa PCO2, OWA group). Scallops were placed in a 4.7 T MR animal scanner and the energetic status of the adductor muscle was determined under resting conditions using in vivo 31P-NMR spectroscopy. The surplus oxidative flux (Qmax) was quantified by recording the recovery of arginine phosphate (PLA) directly after moderate swimming exercise of the scallops. Results:Measurements led to reproducible results within each experimental group. Under projected future conditions resting PLA levels (PLArest) were reduced, indicating reduced energy reserves in warming exposed scallops per se. In comparison to vertebrate muscle tissue surplus Qmax of scallop muscle was about one order of magnitude lower. This can be explained by lower mitochondrial contents and capacities in invertebrate than vertebrate muscle tissue. Warm exposed scallops showed a slower recovery rate of PLA levels (kPLA) and a reduced surplus Qmax. Elevated PCO2 did not affected PLA recovery further. Dataset Arctic North Atlantic Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science Arctic |