Seawater carbonate chemistry and in vivo 31P-MRS of muscle bioenergetics in marine invertebrates

• Main Authors: Bock, Christian, Wermter, Felizitas Charlotte, Schalkhausser, Burgel, Blicher, Martin E, Pörtner, Hans-Otto, Lannig, Gisela, Sejr, Mikael K
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: Alkalinity; total; Animalia; Aragonite saturation state; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Chlamys islandica; Coast and continental shelf; Exponential rate constant for recovery; standard deviation; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Halftime of recovery; Laboratory experiment; Maximal surplus oxidative flux; adenosine triphosphate per time; Mollusca; North Atlantic; Number; Number of claps; OA-ICC; Ocean Acidification International Coordination Centre; Other studied parameter or process; Partial pressure of carbon dioxide; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pecten maximus; pH; Phosphate; inorganic/phospho-L-arginine ratio; Phospho-L-arginine; Arctic; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.959731; https://doi.org/10.1594/PANGAEA.959731

Object：Dynamic in vivo 31P-NMR spectroscopy in combination with Magnetic Resonance Imaging (MRI) was used to study muscle bioenergetics of boreal and Arctic scallops (Pecten maximus and Chlamys islandica) to test the hypothesis that future Ocean Warming and Acidification (OWA) will impair the performance of marine invertebrates. Materials & methods： Experiments were conducted following the recommendations for studies of muscle bioenergetics in vertebrates. Animals were long-term incubated under different environmental conditions: controls at 0 °C for C. islandica and 15 °C for P. maximus under ambient PCO2 of 0.039 kPa, a warm exposure with +5 °C (5 °C and 20 °C, respectively) under ambient PCO2 (OW group), and a combined exposure to warmed acidified conditions (5 °C and 20 °C, 0.112 kPa PCO2, OWA group). Scallops were placed in a 4.7 T MR animal scanner and the energetic status of the adductor muscle was determined under resting conditions using in vivo 31P-NMR spectroscopy. The surplus oxidative flux (Qmax) was quantified by recording the recovery of arginine phosphate (PLA) directly after moderate swimming exercise of the scallops. Results：Measurements led to reproducible results within each experimental group. Under projected future conditions resting PLA levels (PLArest) were reduced, indicating reduced energy reserves in warming exposed scallops per se. In comparison to vertebrate muscle tissue surplus Qmax of scallop muscle was about one order of magnitude lower. This can be explained by lower mitochondrial contents and capacities in invertebrate than vertebrate muscle tissue. Warm exposed scallops showed a slower recovery rate of PLA levels (kPLA) and a reduced surplus Qmax. Elevated PCO2 did not affected PLA recovery further.