Intracellular trace metals content of iron and manganese of Phaeocystis antarctica after exposure to different Fe and Mn availabilities at the end of the experiment

• Main Authors: Balaguer, Jenna, Thoms, Silke, Trimborn, Scarlett
• Format: Dataset
• Language: English
• Published: PANGAEA 2023
• Subjects: ANT-XXXIII/3; carbon fixation; Drake Passage; Fe:C ratio; growth; ICP-MS; Inductively coupled plasma mass spectrometry; In situ pump; iron; cellular quota; ISP; manganese; photophysiology; Phytoplankton; Polarstern; PS112; PS112_26-1; Southern Ocean; Trace metal; Treatment; Antarc*; Antarctica
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.944457; https://doi.org/10.1594/PANGAEA.944457

For determination of the cellular trace metal content, at the end of the experiment 400 mL of water were filtered onto 0.2 μm TMC-cleaned polycarbonate filters (EMD Millipore, Darmstadt, Germany) and then rinsed for 15 min with a 0.1 M oxalic acid wash to remove cell surface bound trace metals. Finally, the filters were rinsed with filtered seawater and stored in 5 mL TMC polyfluoralkoxy vials until further analysis. Samples were analyzed with an inductively coupled plasma mass spectrometry (ICP-MS) following a digestion with nitric acid (HNO3) and HF.