Intracellular trace metals content of iron and manganese of Phaeocystis antarctica after exposure to different Fe and Mn availabilities at the end of the experiment

For determination of the cellular trace metal content, at the end of the experiment 400 mL of water were filtered onto 0.2 μm TMC-cleaned polycarbonate filters (EMD Millipore, Darmstadt, Germany) and then rinsed for 15 min with a 0.1 M oxalic acid wash to remove cell surface bound trace metals. Fina...

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Bibliographic Details
Main Authors: Balaguer, Jenna, Thoms, Silke, Trimborn, Scarlett
Format: Dataset
Language:English
Published: PANGAEA 2022
Subjects:
ANT-XXXIII/3
carbon fixation
Drake Passage
Fe:C ratio
growth
ICP-MS
Inductively coupled plasma mass spectrometry
In situ pump
iron
cellular quota
ISP
manganese
photophysiology
Phytoplankton
Polarstern
PS112
PS112_26-1
Southern Ocean
Trace metal
Treatment
Antarc*
Antarctica
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.944457
Description
Summary:For determination of the cellular trace metal content, at the end of the experiment 400 mL of water were filtered onto 0.2 μm TMC-cleaned polycarbonate filters (EMD Millipore, Darmstadt, Germany) and then rinsed for 15 min with a 0.1 M oxalic acid wash to remove cell surface bound trace metals. Finally, the filters were rinsed with filtered seawater and stored in 5 mL TMC polyfluoralkoxy vials until further analysis. Samples were analyzed with an inductively coupled plasma mass spectrometry (ICP-MS) following a digestion with nitric acid (HNO3) and HF.

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