Phytoplankton pigment concentrations measured by HPLC in the surface water of the Mackenzie Delta Region during 4 expeditions from spring to fall in 2019

• Main Authors: Matsuoka, Atsushi, Juhls, Bennet, Bécu, Guislain, Oziel, Laurent, Leymarie, Edouard, Lizotte, Martine, Ferland, Joannie, Doxaran, David, Maury, Juliette, Béguin, Marine, Laberge-Carignan, Audrey, Guilmette, Caroline, Hilborn, Andrea, Tisserand, Lucas, Devred, Emmanuel, Bossé-Demers, Thomas, Mannino, Antonio, Babin, Marcel
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: 1_STN01; 1_STN020; 1_STN040; 1_STN0a; 1_STN0b; 1_STN140alt; 1_STN150alt; 1_STN340alt; 1_STN350; 1_STN360; 1_STN370alt; 1_STN380alt; 1_STN540alt; 1_STN550; 1_STN740; 1_STN810; 1_STN830; 1_STN840; 1_STN850; 1_STN860; 1_STN870; 19-Butanoyloxyfucoxanthin; 19-Hexanoyloxyfucoxanthin; 2_STN030; 2_STN040; 2_STN1030; 2_STN1040; 2_STN1050; 2_STN1060; 2_STN110; 2_STN120; 2_STN140alt; 2_STN150alt; 2_STN310; 2_STN320; 2_STN330; 2_STN340alt; 2_STN350; 2_STN360; 2_STN370; 2_STN380alt_2; 2_STN420; 2_STN430; 2_STN450; 2_STN530; 2_STN540alt; 2_STN550; 2_STN565; 2_STN620; 2_STN630; Arctic; Mackenzie Delta
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.937585; https://doi.org/10.1594/PANGAEA.937585

The determination of phytoplankton using high performance liquid chromatography (HPLC) is detailed in Hooker et al. (2005; doi:10.1364/AO.44.000553) and Ras et al. (2008; doi:10.5194/bg-5-353-2008). Briefly, the pigments of particles retained on GF/F (0.7 µm) filters were extracted at -20°C with 3 mL methanol (100%). The filter samples were then disrupted using a sonicator and clarified one hour later by vacuum filtration through GF/F filters. The extracts were rapidly analyzed within 24h by HPLC with a complete Agilent Technologies system (comprising LC Chemstation software, a degasser, a binary pump, a refrigerated autosampler, a column thermostat and a diode array detector).