Seawater carbonate chemistry and ROS and EPS production of the Trichodesmium erythraeum

• Main Authors: Wu, Shijie, Mi, Tiezhu, Zhen, Yu, Yu, Elizabeth K, Wang, Fuwen, Yu, Zhigang, Mock, Timothy D
• Format: Dataset
• Language: English
• Published: PANGAEA 2021
• Subjects: Alkalinity; total; standard deviation; Aragonite saturation state; Bacteria; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; organic; particulate; per cell; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Catalase activity; per protein mass; Chlorophyll a; Chlorophyll a per cell; Cyanobacteria; Exopolysaccharides; Experiment duration; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Growth rate; Laboratory experiment; Laboratory strains; Maximum photochemical quantum yield of photosystem II; Nitrogen; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.930305; https://doi.org/10.1594/PANGAEA.930305

The diazotrophic cyanobacterium Trichodesmium is thought to be a major contributor to the new N in the parts of the oligotrophic, subtropical and tropical oceans. In this study physiological and biochemical methods and transcriptome sequencing were used to investigate the influences of ocean acidification (OA) on Trichodesmium erythraeum (T. erythraeum). We presented evidence that OA caused by CO2 slowed the growth rate and physiological activity of T. erythraeum. OA led to reduced development of proportion of the vegetative cells into diazocytes which included up‐regulated genes of nitrogen fixation. Reactive oxygen species (ROS) accumulation was increased due to the disruption of photosynthetic electron transport and decrease in antioxidant enzyme activities under acidified conditions. This study showed that OA increased the amounts of (exopolysaccharides) EPS in T. erythraeum, and the key genes of ribose‐5‐phosphate (R5P) and glycosyltransferases (Tery_3818) were up‐regulated. These results provide new insight into how ROS and EPS of T. erythraeum increase in an acidified future ocean to cope with OA‐imposed stress.