Seawater carbonate chemistry and ROS and EPS production of the Trichodesmium erythraeum

The diazotrophic cyanobacterium Trichodesmium is thought to be a major contributor to the new N in the parts of the oligotrophic, subtropical and tropical oceans. In this study physiological and biochemical methods and transcriptome sequencing were used to investigate the influences of ocean acidifi...

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Bibliographic Details
Main Authors: Wu, Shijie, Mi, Tiezhu, Zhen, Yu, Yu, Elizabeth K, Wang, Fuwen, Yu, Zhigang, Mock, Timothy D
Format: Dataset
Language:English
Published: PANGAEA 2021
Subjects:
Alkalinity
total
standard deviation
Aragonite saturation state
Bacteria
Bicarbonate ion
Bottles or small containers/Aquaria (<20 L)
Calcite saturation state
Calculated using CO2SYS
Calculated using seacarb after Nisumaa et al. (2010)
Carbon
inorganic
dissolved
organic
particulate
per cell
Carbonate ion
Carbonate system computation flag
Carbon dioxide
Catalase activity
per protein mass
Chlorophyll a
Chlorophyll a per cell
Cyanobacteria
Exopolysaccharides
Experiment duration
Fugacity of carbon dioxide (water) at sea surface temperature (wet air)
Growth rate
Laboratory experiment
Laboratory strains
Maximum photochemical quantum yield of photosystem II
Nitrogen
Ocean acidification
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.930305
https://doi.org/10.1594/PANGAEA.930305
Description
Summary:The diazotrophic cyanobacterium Trichodesmium is thought to be a major contributor to the new N in the parts of the oligotrophic, subtropical and tropical oceans. In this study physiological and biochemical methods and transcriptome sequencing were used to investigate the influences of ocean acidification (OA) on Trichodesmium erythraeum (T. erythraeum). We presented evidence that OA caused by CO2 slowed the growth rate and physiological activity of T. erythraeum. OA led to reduced development of proportion of the vegetative cells into diazocytes which included up‐regulated genes of nitrogen fixation. Reactive oxygen species (ROS) accumulation was increased due to the disruption of photosynthetic electron transport and decrease in antioxidant enzyme activities under acidified conditions. This study showed that OA increased the amounts of (exopolysaccharides) EPS in T. erythraeum, and the key genes of ribose‐5‐phosphate (R5P) and glycosyltransferases (Tery_3818) were up‐regulated. These results provide new insight into how ROS and EPS of T. erythraeum increase in an acidified future ocean to cope with OA‐imposed stress.

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