Relative hepatic transcript expression of 48 target genes from post-smolt Atlantic salmon subjected to an incremental temperature increase either under normoxia or hypoxia in a tank-based system

• Main Authors: Beemelmanns, Anne, Zanuzzo, Fabio S, Xue, Xi, Sandrelli, Rebeccah M, Gamperl, A Kurt, Rise, Matthew L
• Format: Dataset
• Language: English
• Published: PANGAEA 2020
• Subjects: Aquaculture; Climate change; hypoxia; Increasing Temperatures; Transcriptomics; Atlantic salmon
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.913696; https://doi.org/10.1594/PANGAEA.913696

In this study, we measured the relative transcript expression of 45 target genes and 3 normalizer genes in liver samples of 120 Atlantic salmon that were subjected to 3 different climate scenarios in a tank-based system. Atlantic salmon were exposed to following treatments: (1) Control (CT) constant temperature of 12°C and 100 % air saturation; (2) Warm&Normoxic (WN) incremental temperature increase (1°C per week from 12 - 20°C) at 100% air saturation; and (3) Warm&Hypoxic (WH) decrease in oxygen content of 70% air saturation over one week, followed by two weeks of acclimation to this oxygen level, and then incremental temperature increase (1°C per week from 12 - 20°C) at 70% air saturation. Liver samples were taken from 8 fish per treatment group (CT, WN and WH) at 5 different temperature measuring points: 12°C, 16°C, 18°C, 19°C, and 20°C. For each treatment group, we used 8 biological fish replicates (4 fish from 2 tank replicates). The sampling during the simulated seasonal temperature increase was performed 3 days after reaching the temperature level of interest (12°C-initial, 16°C-3days, 18°C-3days, 19°C-3days, 20°C-3days). Once the maximum temperature increase of 20°C was reached, an additional sampling after 4 weeks at 20°C was carried out (20°C-4weeks). After each temperature challenge eight fish per per tank replicate (n = 8, N = 24) were euthanized with 400 mg L^-1 of tricaine-methane-sulfonate. Liver tissues (100 mg per sample) were rapidly dissected from fish, placed in RNase-free 1.5 mL tubes, flash-frozen in liquid nitrogen, and stored at -80°C until RNA extractions were performed. The relative transcript expression values of 45 genes of interest (GOIs) and 3 normalizers were assessed for 8 individual fish samples per 3 treatment groups at 5 sampling time points (n = 8, N = 120). Real-time qPCR Fluidigm Biomark HD system based on 96.96 dynamic arrays (GE-arrays) was employed according to the manufacturer's instructions (Fluidigm, Biomark HD). The transcript levels of 48 genes were measured ...