List of number of microsatellite repeats for fungus specific locus in stands of Cetraria aculeata, Usnea antarctica and U. aurantiacoatra

• Main Author: Lagostina, Elisa
• Format: Dataset
• Language: English
• Published: PANGAEA 2019
• Subjects: Biodiversity; Cetraria aculeata; climate change; Conservation; microsatellites; MigrateN; Parmeliaceae; Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas; SPP1158; Usnea antarctica; Usnea aurantiacoatra; Antarctic; Arctic; Antarc*; Antarctica; Climate change; Sea ice
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.906884; https://doi.org/10.1594/PANGAEA.906884

Eight consistently amplifying markers were used for Cetraria aculeata. Samples of Usnea aurantiacoatra and U. antarctica were genotyped using 21 and 22 microsatellites markers, respectively. Detailed information on primers and PCR amplification can be found in Lutsak et al. (2016) and Lagostina et al. (2017). PCR amplicons were electrophoresed using an Applied Biosystems 3730 sequencer, with the LIZ 500 (C. aculeata)or LIZ 600 (Usnea sp.) size standards (Applied Biosystems, Waltham, Mass., USA). Allele sizes were manually scored using the Geneious 10 microsatellites tool (Kearse et al. 2012). Those SSR markers were used to study the effects of dispersal strategy and phylogeographic history on the population genetic structure of Antarctic lichens.