List of number of microsatellite repeats for fungus specific locus in stands of Cetraria aculeata, Usnea antarctica and U. aurantiacoatra

Eight consistently amplifying markers were used for Cetraria aculeata. Samples of Usnea aurantiacoatra and U. antarctica were genotyped using 21 and 22 microsatellites markers, respectively. Detailed information on primers and PCR amplification can be found in Lutsak et al. (2016) and Lagostina et a...

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Bibliographic Details
Main Author: Lagostina, Elisa
Format: Dataset
Language:English
Published: PANGAEA 2019
Subjects:
Biodiversity
Cetraria aculeata
climate change
Conservation
microsatellites
MigrateN
Parmeliaceae
Priority Programme 1158 Antarctic Research with Comparable Investigations in Arctic Sea Ice Areas
SPP1158
Usnea antarctica
Usnea aurantiacoatra
Antarctic
Arctic
Antarc*
Antarctica
Climate change
Sea ice
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.906884
https://doi.org/10.1594/PANGAEA.906884
Description
Summary:Eight consistently amplifying markers were used for Cetraria aculeata. Samples of Usnea aurantiacoatra and U. antarctica were genotyped using 21 and 22 microsatellites markers, respectively. Detailed information on primers and PCR amplification can be found in Lutsak et al. (2016) and Lagostina et al. (2017). PCR amplicons were electrophoresed using an Applied Biosystems 3730 sequencer, with the LIZ 500 (C. aculeata)or LIZ 600 (Usnea sp.) size standards (Applied Biosystems, Waltham, Mass., USA). Allele sizes were manually scored using the Geneious 10 microsatellites tool (Kearse et al. 2012). Those SSR markers were used to study the effects of dispersal strategy and phylogeographic history on the population genetic structure of Antarctic lichens.

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