Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa
Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, a...
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ftpangaea:oai:pangaea.de:doi:10.1594/PANGAEA.888184 2024-09-15T18:27:47+00:00 Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu LATITUDE: 28.466670 * LONGITUDE: 121.183330 * DATE/TIME START: 2015-06-01T00:00:00 * DATE/TIME END: 2015-06-30T00:00:00 2017 text/tab-separated-values, 1080 data points https://doi.pangaea.de/10.1594/PANGAEA.888184 https://doi.org/10.1594/PANGAEA.888184 en eng PANGAEA Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.888184 https://doi.org/10.1594/PANGAEA.888184 CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess Supplement to: Shi, Wei; Han, Yu; Guo, Cheng; Zhao, Xinguo; Liu, Saixi; Su, Wenhao; Wang, Yichen; Zha, Shanjie; Chai, Xueliang; Liu, Guangxu (2017): Ocean acidification hampers sperm-egg collisions, gamete fusion, and generation of Ca 2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa. Marine Environmental Research, 130, 106-112, https://doi.org/10.1016/j.marenvres.2017.07.016 Alkalinity total standard error Amplitude Animalia Aragonite saturation state Average path velocity Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fertilization success rate Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) dataset 2017 ftpangaea https://doi.org/10.1594/PANGAEA.88818410.1016/j.marenvres.2017.07.016 2024-07-24T02:31:33Z Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations. Dataset Ocean acidification PANGAEA - Data Publisher for Earth & Environmental Science ENVELOPE(121.183330,121.183330,28.466670,28.466670) |
institution |
Open Polar |
collection |
PANGAEA - Data Publisher for Earth & Environmental Science |
op_collection_id |
ftpangaea |
language |
English |
topic |
Alkalinity total standard error Amplitude Animalia Aragonite saturation state Average path velocity Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fertilization success rate Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) |
spellingShingle |
Alkalinity total standard error Amplitude Animalia Aragonite saturation state Average path velocity Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fertilization success rate Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
topic_facet |
Alkalinity total standard error Amplitude Animalia Aragonite saturation state Average path velocity Benthic animals Benthos Bicarbonate ion Bottles or small containers/Aquaria (<20 L) Calcite saturation state Calculated using CO2SYS Calculated using seacarb after Nisumaa et al. (2010) Carbon inorganic dissolved Carbonate ion Carbonate system computation flag Carbon dioxide Coast and continental shelf Curvilinear velocity EXP Experiment Fertilization success rate Fluorescence intensity Fugacity of carbon dioxide (water) at sea surface temperature (wet air) Laboratory experiment Mollusca North Pacific OA-ICC Ocean Acidification International Coordination Centre Other metabolic rates Partial pressure of carbon dioxide (water) at sea surface temperature (wet air) |
description |
Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations. |
format |
Dataset |
author |
Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu |
author_facet |
Shi, Wei Han, Yu Guo, Cheng Zhao, Xinguo Liu, Saixi Su, Wenhao Wang, Yichen Zha, Shanjie Chai, Xueliang Liu, Guangxu |
author_sort |
Shi, Wei |
title |
Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
title_short |
Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
title_full |
Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
title_fullStr |
Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
title_full_unstemmed |
Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa |
title_sort |
seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of ca2+ oscillations of a broadcast spawning bivalve, tegillarca granosa |
publisher |
PANGAEA |
publishDate |
2017 |
url |
https://doi.pangaea.de/10.1594/PANGAEA.888184 https://doi.org/10.1594/PANGAEA.888184 |
op_coverage |
LATITUDE: 28.466670 * LONGITUDE: 121.183330 * DATE/TIME START: 2015-06-01T00:00:00 * DATE/TIME END: 2015-06-30T00:00:00 |
long_lat |
ENVELOPE(121.183330,121.183330,28.466670,28.466670) |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Supplement to: Shi, Wei; Han, Yu; Guo, Cheng; Zhao, Xinguo; Liu, Saixi; Su, Wenhao; Wang, Yichen; Zha, Shanjie; Chai, Xueliang; Liu, Guangxu (2017): Ocean acidification hampers sperm-egg collisions, gamete fusion, and generation of Ca 2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa. Marine Environmental Research, 130, 106-112, https://doi.org/10.1016/j.marenvres.2017.07.016 |
op_relation |
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb https://doi.pangaea.de/10.1594/PANGAEA.888184 https://doi.org/10.1594/PANGAEA.888184 |
op_rights |
CC-BY-3.0: Creative Commons Attribution 3.0 Unported Access constraints: unrestricted info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1594/PANGAEA.88818410.1016/j.marenvres.2017.07.016 |
_version_ |
1810469051226390528 |