Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa

• Main Authors: Shi, Wei, Han, Yu, Guo, Cheng, Zhao, Xinguo, Liu, Saixi, Su, Wenhao, Wang, Yichen, Zha, Shanjie, Chai, Xueliang, Liu, Guangxu
• Format: Dataset
• Language: English
• Published: PANGAEA 2017
• Subjects: Alkalinity; total; standard error; Amplitude; Animalia; Aragonite saturation state; Average path velocity; Benthic animals; Benthos; Bicarbonate ion; Bottles or small containers/Aquaria (<20 L); Calcite saturation state; Calculated using CO2SYS; Calculated using seacarb after Nisumaa et al. (2010); Carbon; inorganic; dissolved; Carbonate ion; Carbonate system computation flag; Carbon dioxide; Coast and continental shelf; Curvilinear velocity; EXP; Experiment; Fertilization success rate; Fluorescence intensity; Fugacity of carbon dioxide (water) at sea surface temperature (wet air); Laboratory experiment; Mollusca; North Pacific; OA-ICC; Ocean Acidification International Coordination Centre; Other metabolic rates; Partial pressure of carbon dioxide (water) at sea surface temperature (wet air); Pacific; Ocean acidification
• Online Access: https://doi.pangaea.de/10.1594/PANGAEA.888184; https://doi.org/10.1594/PANGAEA.888184

Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations.