KOSMOS Finland 2012 mesocosm study: Size-fractionated bacterial protein production (BPP) of free-living and particle associated bacteria and abundance of particle associated heterotrophic prokaryotes.

The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the couplin...

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Bibliographic Details
Main Authors: Hornick, Thomas, Bach, Lennart Thomas, Crawfurd, Katharine J, Spilling, Kristian, Achterberg, Eric Pieter, Woodhouse, Jason N, Schulz, Kai Georg, Brussaard, Corina P D, Riebesell, Ulf, Grossart, Hans-Peter
Format: Dataset
Language:English
Published: PANGAEA 2016
Subjects:
BIOACID
Biological Impacts of Ocean Acidification
DATE/TIME
Day of experiment
KOSMOS_2012_Tvaerminne
MESO
Mesocosm experiment
Mesocosm label
Prokaryotes
heterotroph
particle associated
Protein production
free-living bacteria
particle associated bacteria
SOPRAN
Surface Ocean Processes in the Anthropocene
Ocean acidification
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.868621
https://doi.org/10.1594/PANGAEA.868621
Description
Summary:The oceans absorb about a quarter of the annually produced anthropogenic atmospheric carbon dioxide (CO2), resulting in a decrease in surface water pH, a process termed ocean acidification (OA). Surprisingly little is known about how OA affects the physiology of heterotrophic bacteria or the coupling of heterotrophic bacteria to phytoplankton when nutrients are limited. Previous experiments were, for the most part, undertaken during productive phases or following nutrient additions designed to stimulate algal blooms. Therefore, we performed an in situ large-volume mesocosm (ca. 55 m**3) experiment in the Baltic Sea by simulating different fugacities of CO2 (fCO2) extending from present to future conditions. The study was conducted in July?August after the nominal spring bloom, in order to maintain low-nutrient conditions throughout the experiment. This resulted in phytoplankton communities dominated by small-sized functional groups (picophytoplankton). There was no consistent fCO2-induced effect on bacterial protein production (BPP), cell-specific BPP (csBPP) or biovolumes (BVs) of either free-living (FL) or particle-associated (PA) heterotrophic bacteria, when considered as individual components (univariate analyses). Permutational Multivariate Analysis of Variance (PERMANOVA) revealed a significant effect of the fCO2 treatment on entire assemblages of dissolved and particulate nutrients, metabolic parameters and the bacteria?phytoplankton community. However, distance-based linear modelling only identified fCO2 as a factor explaining the variability observed amongst the microbial community composition, but not for explaining variability within the metabolic parameters. This suggests that fCO2 impacts on microbial metabolic parameters occurred indirectly through varying physicochemical parameters and microbial species composition. Cluster analyses examining the co-occurrence of different functional groups of bacteria and phytoplankton further revealed a separation of the four fCO2-treated mesocosms from both ...

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