Combined metabolome and proteome analysis of the mantle tissue from Pacific oyster Crassostrea gigas exposed to elevated pCO2

Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of C...

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Bibliographic Details
Main Authors: Wei, Lei, Wang, Qing, Ning, Xuanxuan, Mu, Changkao, Wang, Chunlin, Cao, Ruiwen, Wu, Huifeng, Cong, Ming, Li, Fei, Ji, Chenglong, Zhao, Jianmin
Format: Dataset
Language:English
Published: PANGAEA 2015
Subjects:
Alkalinity
total
standard deviation
Animalia
Aragonite saturation state
Benthic animals
Benthos
Bicarbonate ion
Calcite saturation state
Calculated using CO2SYS
Calculated using seacarb after Nisumaa et al. (2010)
Carbon
inorganic
dissolved
Carbonate ion
Carbonate system computation flag
Carbon dioxide
Coast and continental shelf
Containers and aquaria (20-1000 L or < 1 m**2)
Crassostrea gigas
Fugacity of carbon dioxide (water) at sea surface temperature (wet air)
Gene expression (incl. proteomics)
Gene name
Laboratory experiment
Mollusca
mRNA gene expression
relative
OA-ICC
Ocean Acidification International Coordination Centre
Partial pressure of carbon dioxide
Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)
pH
Salinity
Single species
South Pacific
Species
Ocean acidification
Pacific oyster
Online Access:https://doi.pangaea.de/10.1594/PANGAEA.846883
https://doi.org/10.1594/PANGAEA.846883
Description
Summary:Ocean acidification (OA) has been found to affect an array of normal physiological processes in mollusks, especially posing a significant threat to the fabrication process of mollusk shell. In the current study, the impact of exposure to elevated pCO2 condition was investigated in mantle tissue of Crassostrea gigas by an integrated metabolomic and proteomic approach. Analysis of metabolome and proteome revealed that elevated pCO2 could affect energy metabolism in oyster C. gigas, marked by differentially altered ATP, succinate, MDH, PEPCK and ALDH levels. Moreover, the up-regulated calponin-2, tropomyosins and myosin light chains indicated that elevated pCO2 probably caused disturbances in cytoskeleton structure in mantle tissue of oyster C. gigas. This work demonstrated that a combination of proteomics and metabolomics could provide important insights into the effects of OA at molecular levels.

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